Full-Thickness Intraoral Mucosa Barrier Models for In Vitro Drug-Permeation Studies Using Microneedles

Autor(es)

Serpe, Luciano ^[1] ; Muniz, Bruno Vilela ^[1] ; dos Santos, Cleiton Pita ^[1] ; da Silva, Camila Batista ^[1] ; Volpato, Maria Cristina ^[1] ; Groppo, Francisco Carlos ^[1] ; Vianna Lopez, Renata Fonseca ^[2] ; Franz-Montan, Michelle ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, Piracicaba, SP - Brazil

^[2] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Pharmaceutical Sciences; v. 108, n. 5, p. 1756-1764, MAY 2019.
• Citações Web of Science: 1

Resumo

The use of permeation enhancers such as microneedles (MNs) to increase drug penetration across intraoral mucosa has increased in recent years. Permeation studies, commonly performed using vertical diffusion cells, are a well-established way to preview formulations and enhance their performance during the development stage. However, to our knowledge, the existing intraoral mucosa barrier models do not permit permeation using MN-pretreated mucosa due to their insufficient thickness. Therefore, the objective of this study was to develop a barrier model using thick palate tissues to perform in vitro permeation studies, with physical enhancement of the permeability of intraoral mucosa by pretreatment with MNs. The adapted Franz-type cells used in the permeation experiments were validated (cell dimensions and volume, sealing effectiveness, stirring and dissolution efficiency, temperature control, and establishment of uniaxial flux). Commercially available MNs were used in the palatal mucosa. Optical images of the mucosa were acquired to analyze the microperforations created. In vitro permeation studies were conducted with the MN-pretreated mucosa. This work presents a new in vitro method for the evaluation of MNs as permeation enhancers, with the aim of improving the absorption of drug formulations topically applied within the oral cavity. (c) 2019 American Pharmacists Association (R). Published by Elsevier Inc. All rights reserved (AU)

• Processo FAPESP: 12/06974-4 - Estudos de desenvolvimento, biocompatibilidade e permeação de formulações em gel de anestésicos locais associados a nanocápsulas de poli-epsilon-caprolactona
• Beneficiário: Michelle Franz Montan Braga Leite
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 12/02492-5 - Estudo in vitro da aplicabilidade do sistema de microagulhas para anestesia tópica em tecidos orais de suínos.
• Beneficiário: Luciano Serpe
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/18113-0 - Desenvolvimento de metodologias para estudos de permeação de formulações tópicas para uso em odontologia
• Beneficiário: Francisco Carlos Groppo
• Modalidade de apoio: Auxílio à Pesquisa - Regular