Phenotypic characterization and virulence-related properties of Escherichia albertii strains isolated from children with diarrhea in Brazil

Autor(es)

Lima, Mauricio P. ^[1] ; Yamamoto, Denise ^{[1, 2]} ; de Mello Santos, Ana Carolina ^[1] ; Ooka, Tadasuke ^[3] ; Hernandes, Rodrigo T. ^[4] ; Vieira, Monica A. M. ^[1] ; Santos, Fernanda Fernandes ^[1] ; Silva, Rosa Maria ^[1] ; Hayashi, Tetsuya ^[5] ; Gomes, Tania A. T. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Escola Paulista Med UNIFESP EPM, Dept Microbiol Imunol & Parasitol, Rua Botucatu 862, 3 Andar, BR-04023062 Sao Paulo, SP - Brazil

^[2] Univ Santo Amaro UNISA, Rua Prof Eneas de Siqueira Neto, BR-04829300 Sao Paulo, SP - Brazil

^[3] Kagoshima Univ, Grad Sch Med & Dent Sci, Dept Microbiol, Kagoshima - Japan

^[4] Univ Estadual Paulista Julio de Mesquita Filho UN, Inst Biociencias, Dept Microbiol & Imunol, BR-18618691 Botucatu, SP - Brazil

^[5] Kyushu Univ, Fac Med Sci, Dept Bacteriol, Higashi Ku, 3-1-1 Maedashi, Fukuoka, Fukuoka 8128582 - Japan

• Tipo de documento: Artigo Científico
• Fonte: PATHOGENS AND DISEASE; v. 77, n. 2 MAR 2019.
• Citações Web of Science: 0

Resumo

Escherichia albertii are emerging enteropathogens, whose identification is difficult, as they share biochemical characteristics and some virulence-related genes with diarrheagenic Escherichia coli (DEC). Studies on phylogeny, phenotypic characteristics and potential virulence factors of human E. albertii strains are scarce. In this study, we identified by multiplex PCR five E. albertii among 106 strains isolated from diarrheic children in Sao Paulo, Brazil, which were previously classified as atypical enteropathogenic E. coli. All strains were investigated regarding their phylogeny, biochemical properties, virulence-related properties, antimicrobial resistance and presence of putative virulence-related genes. All strains belonged to different E. albertii lineages and adhered to and produced attaching and effacing lesions on HeLa cells. Three strains invaded Caco-2 cells, but did not persist intracellularly, and three formed biofilms on polystyrene surfaces. All strains were resistant to few antibiotics and only one carried a self-transmissible resistance plasmid. Finally, among 38 DEC and 18 extraintestinal pathogenic E. coli (ExPEC) virulence-related genes searched, six and three were detected, respectively, with paa and cdtB being found in all strains. Despite the limited number of strains, this study provided additional knowledge on human E. albertii virulence potential, showing that they share important virulence factors with DEC and ExPEC. (AU)

• Processo FAPESP: 11/12664-5 - Explorando as interações de Escherichia coli enteropatogênica com células intestinais in vitro e in vivo
• Beneficiário: Tânia Aparecida Tardelli Gomes do Amaral
• Modalidade de apoio: Auxílio à Pesquisa - Regular