Identification of Cell-Free Circulating MicroRNAs for the Detection of Early Breast Cancer and Molecular Subtyping

Autor(es):Mostrar menos -

Souza, Karen C. B. ^[1] ; Evangelista, Adriane F. ^[1] ; Leal, Leticia F. ^[1] ; Souza, Cristiano P. ^[2] ; Vieira, Rene A. ^[3] ; Causin, Rhafaela L. ^[1] ; Neuber, A. C. ^[4] ; Pessoa, Daniele P. ^[1] ; Passos, Geraldo A. S. ^[5] ; Reis, Rui M. V. ^{[6, 1, 7]} ; Marques, Marcia M. C. ^{[1, 4, 8]}

Afiliação do(s) autor(es)

^[1] Barretos Canc Hosp, Mol Oncol Res Ctr, Barretos - Brazil

^[2] Barretos Canc Hosp, Dept Clin Oncol, Barretos, SP - Brazil

^[3] Barretos Canc Hosp, Dept Mastol & Breast Reconstruct, Barretos - Brazil

^[4] Barretos Canc Hosp, Tumor Biobank, Barretos, SP - Brazil

^[5] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Basic & Oral Biol, Sao Paulo - Brazil

^[6] ICVS 3Bs PT Govt Associate Lab, Braga - Portugal

^[7] Univ Minho, Hlth Sci Sch, Life & Hlth Sci Res Inst ICVS, Braga - Portugal

^[8] FACISB, Barretos Sch Hlth Sci, Barretos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ONCOLOGY; v. 2019, AUG 8 2019.
• Citações Web of Science: 0

Resumo

Early detection is crucial for achieving a reduction in breast cancer mortality. Analysis of circulating cell-free microRNAs present in the serum of cancer patients has emerged as a promising new noninvasive biomarker for early detection of tumors and for predicting their molecular classifications. The rationale for this study was to identify subtype-specific molecular profiles of cell-free microRNAs for early detection of breast cancer in serum. Fifty-four early-stage breast cancers with 27 age-matched controls were selected for circulating microRNAs evaluation in the serum. The 54 cases were molecularly classified (luminal A, luminal B, luminal B Her2 positive, Her-2, triple negative). NanoString platform was used for digital detection and quantitation of 800 tagged microRNA probes and comparing the overall differences in serum microRNA expression from breast cancer cases with controls. We identified the 42 most significant (P <= 0.05, 1.5-fold) differentially expressed circulating microRNAs in each molecular subtype for further study. Of these microRNAs, 19 were significantly differentially expressed in patients presenting with luminal A, eight in the luminal B, ten in luminal B HER 2 positive, and four in the HER2 enriched subtype. AUC is high with suitable sensitivity and specificity. For the triple negative subtype miR-25-3p had the best accuracy. Predictive analysis of the mRNA targets suggests they encode proteins involved in molecular pathways such as cell adhesion, migration, and proliferation. This study identified subtype-specific molecular profiles of cell-free microRNAs suitable for early detection of breast cancer selected by comparison to the microRNA profile in serum for female controls without apparent risk of breast cancer. This molecular profile should be validated using larger cohort studies to confirm the potential of these miRNA for future use as early detection biomarkers that could avoid unnecessary biopsy in patients with a suspicion of breast cancer. (AU)

• Processo FAPESP: 15/21082-0 - Assinaturas moleculares de microRNAs circulantes como marcadores não invasivos para detecção precoce do câncer de mama.
• Beneficiário: Márcia Maria Chiquitelli Marques Silveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular