Physiological concentrations of beta-hydroxybutyrate do not promote adipocyte browning

Autor(es)

Caminhotto, Rennan de Oliveira ^[1] ; Andreotti, Sandra ^[1] ; Medeiros Komino, Ayumi Cristina ^[1] ; Silva, Flaviane de Fatima ^[1] ; Sertie, Rogerio Antonio Laurato ^[2] ; Christoffolete, Marcelo Augusto ^[3] ; Reis, Gabriela Boltes ^[1] ; Lima, Fabio Bessa ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, Sao Paulo - Brazil

^[2] Univ Alabama Birmingham, Dept Nutr Sci, Birmingham, AL 35294 - USA

^[3] Fed Univ ABC, Ctr Nat & Human Sci, Santo Andre - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Life Sciences; v. 232, SEP 1 2019.
• Citações Web of Science: 0

Resumo

Aims: Previous work has demonstrated that ketogenic diets promote white fat browning; however, the exact mechanisms underlying this phenomenom have yet to be elucidated. Recently, an in vitro study showed that supraphysiological concentrations of beta-hydroxybutyrate (beta HB) had a strong influence on the induction of adipocyte browning. On the other hand, concentrations in the physiological range, achieved through ketogenic diets and prolonged fasting produce values of 1-3 mM and 4-7 mM, respectively. Herein, we investigated the impact of physiological concentrations of beta HB on metabolism, and the expression of uncoupling protein 1 (UCP1) and other browning markers in adipose tissues. Main methods: The effects of beta HB on adipocyte browning were investigated in vitro, using primary cultures of isolated visceral and subcutaneous fat cells and cultured 3T3-L1 adipocytes, and in vivo. Key findings: It was determined that beta HB failed to induce changes in the oxidative capacity, citrate synthase activity or browning gene expression patterns in isolated adipocytes, and did not exert a permissive effect on beta-adrenergic agonist-induced browning. In addition, 3T3-L1 adipocytes differentiated following beta HB treatment exhibited downregulated Ucpl expression levels, a result that was recapitulated in the subcutaneous adipose tissue of Wistar rats after beta HB salt treatment. Rats administered beta HB salts also presented reduced brown adipose tissue UCP1 protein expression. Significance: The mechanisms underlying ketogenic diet-induced browning of adipocytes are not known. The results from the present study indicate that physiological concentrations of beta HB are not responsible for this phenomenon, despite the observed beta HB-mediated downregulation of UCP1 expression. (AU)

• Processo FAPESP: 14/10705-4 - Efeito do beta-hidroxibutirato na função mitocondrial de adipócitos brancos isolados
• Beneficiário: Rennan de Oliveira Caminhotto
• Modalidade de apoio: Bolsas no Brasil - Doutorado