Positive influence of simvastatin used as adjuvant agent for cavity lining

Autor(es)

Leite, Maria Luisa ^[1] ; Soares, Diana Gabriela ^[2] ; de Oliveira Duque, Carla Caroline ^[1] ; Ferreira Bordini, Ester Alves ^[1] ; Anovazzi, Giovana ^[3] ; Basso, Fernanda Goncalves ^[4] ; Palomari Spolidorio, Denise Madalena ^[4] ; Hebling, Josimeri ^[3] ; de Souza Costa, Carlos Alberto ^[4]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ Unesp, Araraquara Sch Dent, Dept Dent Mat & Prosthodont, Rua Humaita 1680, BR-14801903 Araraquara, SP - Brazil

^[2] Univ Sao Paulo, Bauru Sch Dent, Dept Operat Dent Endodont & Dent Mat, Rua Alameda Dr Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

^[3] Sao Paulo State Univ Unesp, Araraquara Sch Dent, Dept Orthodont & Pediat Dent, Rua Humaita 1680, BR-14801903 Araraquara, SP - Brazil

^[4] Sao Paulo State Univ Unesp, Araraquara Sch Dent, Dept Physiol & Pathol, Rua Humaita 1680, BR-14801903 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CLINICAL ORAL INVESTIGATIONS; v. 23, n. 9, p. 3457-3469, SEP 2019.
• Citações Web of Science: 0

Resumo

Objectives To assess the biological, antimicrobial, and mechanical effects of the treatment of deep dentin with simvastatin (SV) before application of a glass-ionomer cement (GIC). Materials and methods Dentin discs were adapted to artificial pulp chambers and SV (2.5 or 1.0 mg/mL) was applied to the occlusal surface, either previously conditioned or not with EDTA (+/- EDTA). The extracts (culture medium + SV that diffused through dentin) was obtained and then applied to cultured odontoblast-like MDPC-23 cells. Cell viability, alkaline phosphatase (ALP) activity, and mineralization nodule (MN) deposition were evaluated. Untreated discs were used as control. The antibacterial activity of SV (2.5 or 1.0 mg/mL) against Streptococcus mutans and Lactobacillus acidophilus, as well as the bond strength of GIC to dentin in the presence of SV 2.5 mg/mL (+/- EDTA) were also assessed. The data were analyzed by ANOVA/Tukey tests (alpha = 5%). Results EDTA + SV 2.5 mg/mL significantly enhanced the ALP activity and MN deposition in comparison with the control, without changing in the cell viability (p < 0.05). The association EDTA + SV 2.5 mg/mL + GIC determined the highest ALP and MN values (p < 0.05). SV presented intense antimicrobial activity, and the EDTA dentin conditioning followed by SV application increased bond strength values compared with SV treatment alone (p < 0.05). Conclusion SV presents antimicrobial activity and diffuses across conditioned dentin to biostimulate odontoblast-like pulp cells. (AU)

• Processo FAPESP: 15/15635-7 - Bioestimulação de células pulpares pela adição de sinvastatina em cimentos de ionômero de vidro
• Beneficiário: Maria Luísa de Alencar e Silva Leite
• Modalidade de apoio: Bolsas no Brasil - Mestrado