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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Positive influence of simvastatin used as adjuvant agent for cavity lining

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Author(s):
Leite, Maria Luisa [1] ; Soares, Diana Gabriela [2] ; de Oliveira Duque, Carla Caroline [1] ; Ferreira Bordini, Ester Alves [1] ; Anovazzi, Giovana [3] ; Basso, Fernanda Goncalves [4] ; Palomari Spolidorio, Denise Madalena [4] ; Hebling, Josimeri [3] ; de Souza Costa, Carlos Alberto [4]
Total Authors: 9
Affiliation:
[1] Sao Paulo State Univ Unesp, Araraquara Sch Dent, Dept Dent Mat & Prosthodont, Rua Humaita 1680, BR-14801903 Araraquara, SP - Brazil
[2] Univ Sao Paulo, Bauru Sch Dent, Dept Operat Dent Endodont & Dent Mat, Rua Alameda Dr Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil
[3] Sao Paulo State Univ Unesp, Araraquara Sch Dent, Dept Orthodont & Pediat Dent, Rua Humaita 1680, BR-14801903 Araraquara, SP - Brazil
[4] Sao Paulo State Univ Unesp, Araraquara Sch Dent, Dept Physiol & Pathol, Rua Humaita 1680, BR-14801903 Araraquara, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: CLINICAL ORAL INVESTIGATIONS; v. 23, n. 9, p. 3457-3469, SEP 2019.
Web of Science Citations: 0
Abstract

Objectives To assess the biological, antimicrobial, and mechanical effects of the treatment of deep dentin with simvastatin (SV) before application of a glass-ionomer cement (GIC). Materials and methods Dentin discs were adapted to artificial pulp chambers and SV (2.5 or 1.0 mg/mL) was applied to the occlusal surface, either previously conditioned or not with EDTA (+/- EDTA). The extracts (culture medium + SV that diffused through dentin) was obtained and then applied to cultured odontoblast-like MDPC-23 cells. Cell viability, alkaline phosphatase (ALP) activity, and mineralization nodule (MN) deposition were evaluated. Untreated discs were used as control. The antibacterial activity of SV (2.5 or 1.0 mg/mL) against Streptococcus mutans and Lactobacillus acidophilus, as well as the bond strength of GIC to dentin in the presence of SV 2.5 mg/mL (+/- EDTA) were also assessed. The data were analyzed by ANOVA/Tukey tests (alpha = 5%). Results EDTA + SV 2.5 mg/mL significantly enhanced the ALP activity and MN deposition in comparison with the control, without changing in the cell viability (p < 0.05). The association EDTA + SV 2.5 mg/mL + GIC determined the highest ALP and MN values (p < 0.05). SV presented intense antimicrobial activity, and the EDTA dentin conditioning followed by SV application increased bond strength values compared with SV treatment alone (p < 0.05). Conclusion SV presents antimicrobial activity and diffuses across conditioned dentin to biostimulate odontoblast-like pulp cells. (AU)

FAPESP's process: 15/15635-7 - BIOSTIMULATION OF PULP CELLS BY ADDITION OF SIMVASTATIN IN GLASS IONOMER CEMENTS
Grantee:Maria Luísa de Alencar e Silva Leite
Support Opportunities: Scholarships in Brazil - Master