Positive influence of simvastatin used as adjuvant agent for cavity lining

Author(s)

Leite, Maria Luisa ^[1] ; Soares, Diana Gabriela ^[2] ; de Oliveira Duque, Carla Caroline ^[1] ; Ferreira Bordini, Ester Alves ^[1] ; Anovazzi, Giovana ^[3] ; Basso, Fernanda Goncalves ^[4] ; Palomari Spolidorio, Denise Madalena ^[4] ; Hebling, Josimeri ^[3] ; de Souza Costa, Carlos Alberto ^[4]

Affiliation

^[1] Sao Paulo State Univ Unesp, Araraquara Sch Dent, Dept Dent Mat & Prosthodont, Rua Humaita 1680, BR-14801903 Araraquara, SP - Brazil

^[2] Univ Sao Paulo, Bauru Sch Dent, Dept Operat Dent Endodont & Dent Mat, Rua Alameda Dr Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

^[3] Sao Paulo State Univ Unesp, Araraquara Sch Dent, Dept Orthodont & Pediat Dent, Rua Humaita 1680, BR-14801903 Araraquara, SP - Brazil

^[4] Sao Paulo State Univ Unesp, Araraquara Sch Dent, Dept Physiol & Pathol, Rua Humaita 1680, BR-14801903 Araraquara, SP - Brazil

• Document type: Journal article
• Source: CLINICAL ORAL INVESTIGATIONS; v. 23, n. 9, p. 3457-3469, SEP 2019.
• Web of Science Citations: 0

Abstract

Objectives To assess the biological, antimicrobial, and mechanical effects of the treatment of deep dentin with simvastatin (SV) before application of a glass-ionomer cement (GIC). Materials and methods Dentin discs were adapted to artificial pulp chambers and SV (2.5 or 1.0 mg/mL) was applied to the occlusal surface, either previously conditioned or not with EDTA (+/- EDTA). The extracts (culture medium + SV that diffused through dentin) was obtained and then applied to cultured odontoblast-like MDPC-23 cells. Cell viability, alkaline phosphatase (ALP) activity, and mineralization nodule (MN) deposition were evaluated. Untreated discs were used as control. The antibacterial activity of SV (2.5 or 1.0 mg/mL) against Streptococcus mutans and Lactobacillus acidophilus, as well as the bond strength of GIC to dentin in the presence of SV 2.5 mg/mL (+/- EDTA) were also assessed. The data were analyzed by ANOVA/Tukey tests (alpha = 5%). Results EDTA + SV 2.5 mg/mL significantly enhanced the ALP activity and MN deposition in comparison with the control, without changing in the cell viability (p < 0.05). The association EDTA + SV 2.5 mg/mL + GIC determined the highest ALP and MN values (p < 0.05). SV presented intense antimicrobial activity, and the EDTA dentin conditioning followed by SV application increased bond strength values compared with SV treatment alone (p < 0.05). Conclusion SV presents antimicrobial activity and diffuses across conditioned dentin to biostimulate odontoblast-like pulp cells. (AU)

• FAPESP's process: 15/15635-7 - BIOSTIMULATION OF PULP CELLS BY ADDITION OF SIMVASTATIN IN GLASS IONOMER CEMENTS
• Grantee: Maria Luísa de Alencar e Silva Leite
• Support Opportunities: Scholarships in Brazil - Master