BIOSTIMULATION OF PULP CELLS BY ADDITION OF SIMVASTATIN IN GLASS IONOMER CEMENTS

• Grant number: 15/15635-7
• Support type: Scholarships in Brazil - Master
• Effective date (Start): October 01, 2015
• Effective date (End): February 28, 2017
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal researcher: Carlos Alberto de Souza Costa
• Grantee: Maria Luísa de Alencar e Silva Leite
• Home Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

The stimulation of the dental pulp regenerative process by modulating cell responses is a very important characteristic of dental materials used on deep dentin and exposed pulp treatment. Therefore, the aim of this project is to evaluate the effectiveness of simvastatin incorporation to glass ionomer cements (GICs) on odontoblast-like cells secretive activity stimulation. Initially, a bioactive and cytocompatible concentration of simvastatin will be determined (0.01 µM/L; 0.1 µM/L; 1 µM/L), by mensurating the cell viability (Alamar Blue), alkaline phosphatase (ALP) activity (thymolphthalein monophosphate) and mineralized matrix deposition (Alizarin Red) of odontoblast-like MDPC-23 cells. According to the results, the simvastatin will be incorporated to conventional and resin-modified GICs and its release in humid environment will be assessed (absorbance 428 nm). Then, the bioactivity to the odontoblast-like cells of the GICs containing or not simvastatin will be assessed. For that, the MDPC-23 cells will be seeded on the lower membrane of transwells with 3um poro size (simulating the size of dentin tubules at deep dentin), and the materials will be applied on the upper membrane surface. The cell viability, ALP activity and mineralized nodule deposition will be evaluated at different time-points. The data will be analyzed regarding normality and homoscedasticity in order to select the statistics tests (p = 0.05).

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LEITE, MARIA LUISA; SOARES, DIANA GABRIELA; DE OLIVEIRA DUQUE, CARLA CAROLINE; FERREIRA BORDINI, ESTER ALVES; ANOVAZZI, GIOVANA; BASSO, FERNANDA GONCALVES; PALOMARI SPOLIDORIO, DENISE MADALENA; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Positive influence of simvastatin used as adjuvant agent for cavity lining. CLINICAL ORAL INVESTIGATIONS, v. 23, n. 9, p. 3457-3469, SEP 2019. Web of Science Citations: 0.

DE ALENCAR E SILVA LEITE, MARIA LUISA; SOARES, DIANA GABRIELA; BASSO, FERNANDA GONCALVES; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Biostimulatory effects of simvastatin on MDPC-23 odontoblast-like cells. Brazilian Oral Research, v. 31, p. -, 2017. Web of Science Citations: 0.