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| Author(s): |
Maria Luísa de Alencar e Silva Leite
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Araraquara. 2017-03-21. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araraquara |
| Defense date: | 2017-02-23 |
| Advisor: | Carlos Alberto de Souza Costa; Diana Gabriela de Sousa Soares |
| Abstract | |
The aim of the present study was to determine whether the treatment of dentin with simvastatin (SV) before application of glass-ionomer cement (GIC), allows transdentinal diffusion of this drug at bioactive concentration capable of increasing the potential of odontoblast-like cells to deposit and mineralize dentin matrix. In the 1st study, the dose-response and time-course of SV were determined for the odontoblast-like MDPC-23 cells. Two concentrations (0.01 and 0.1 μM) of SV and three treatment times (24 h, 72 h and continuous – up to 14 days) were evaluated. In the 2nd study, the SV transdentinal diffusion hypothesis was confirmed by liquid chromatography mass spectrometry (LC-MS / MS). Then, concentrations of 2.5 and 1.0 mg/ml were selected to evaluate the transdentinal bioactivity of this drug with MDPC-23 cells, when dentin was treated or not with EDTA. Also in this study the antimicrobial activity (disc diffusion assay) of 2.5 and 1.0 mg/ml SV against Streptococcus mutans and Lactobacillus acidophilus was evaluated. In the 3rd study, the 2.5 mg/mL SV was selected to analyze the bioactivity of dentin treatment with SV followed by lining with Ketac Molar (3M ESPE). In all the studies, the following cell parameters were analyzed: cell viability (MTT), ALP activity (thymolphthalein monophosphate) and mineralized matrix deposition (alizarin red) (n=6). The concentration of 2.5 mg/ml SV was selected to evaluate dentin microshear-bond strength of GIC Ketac Molar when applied on dentin conditioned or not with EDTA. Data were submitted to ANOVA and Tukey test’s (p<0.05). The results of the 1st study demonstrated that exposing MDPC-23 to low concentrations of SV during short periods of time resulted in a bioactive effect associated with no toxicity to cultured cells. In the transdentinal study, it was detected an increase on ALP activity and mineralized matrix deposition when dentin was treated with SV; however, only SV 2.5 mg/ml, associated or not to EDTA, resulted in values significantly higher than negative control for both cell parameters. Finally, it was observed that lining dentin with GIC associated with pre-treatment with EDTA and SV 2.5 mg/ml resulted in the best cell responses regarding the expression of odontoblastic phenotypic markers. In addition, SV featured antimicrobial activity at both tested concentrations against the bacterial strains evaluated. Finally, pre-treatment of dentin with EDTA + SV resulted in significantly higher bond strength values compared to SV treatment. Therefore, based upon the methodology employed in the present in vitro, it was possible to concluded that the treatment of dentin substrate with a specific concentration of simvastatin before application of glass-ionomer cement enhances the capability of odontoblast-like cells to deposit calcium-rich mineralized matrix (AU) | |
| FAPESP's process: | 15/15635-7 - BIOSTIMULATION OF PULP CELLS BY ADDITION OF SIMVASTATIN IN GLASS IONOMER CEMENTS |
| Grantee: | Maria Luísa de Alencar e Silva Leite |
| Support Opportunities: | Scholarships in Brazil - Master |