The pH Signaling Transcription Factor PAC-3 Regulates Metabolic and Developmental Processes in Pathogenic Fungi

Autor(es)

Martins, Maira Pompeu ^[1] ; Martinez-Rossi, Nilce M. ^[1] ; Sanches, Pablo R. ^[1] ; Gomes, Eriston Vieira ^[2] ; Bertolini, Maria Celia ^[3] ; Pedersoli, Wellington R. ^[1] ; Silva, Roberto Nascimento ^[4] ; Rossi, Antonio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, Ribeirao Preto - Brazil

^[2] Morgana Potrich Coll, Dept Biofunct, Mineiros - Brazil

^[3] Sao Paulo State Univ, Inst Chem, Dept Biochem & Technol Chem, UNESP, Araraquara - Brazil

^[4] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Biochem & Immunol, Ribeirao Preto - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN MICROBIOLOGY; v. 10, SEP 4 2019.
• Citações Web of Science: 0

Resumo

The zinc finger transcription factor PAC-3/RIM101/PacC has a defined role in the secretion of enzymes and proteins in response to ambient pH, and also contributes to the virulence of species. Herein we evaluated the role of PAC-3 in the regulation of Neurospora crassa genes, in a model that examined the plant-fungi interactions. N. crassa is a model fungal species capable of exhibiting dynamic responses to its environment by employing endophytic or phytopathogenic behavior according to a given circumstance. Since plant growth and productivity are highly affected by pH and phosphorus (P) acquisition, we sought to verify the impact that induction of a Delta pac-3 mutation would have under limited and sufficient Pi availability, while ensuring that the targeted physiological adjustments mimicked ambient pH and nutritional conditions required for efficient fungal growth and development. Our results suggest direct regulatory functions for PAC-3 in cell wall biosynthesis, homeostasis, oxidation-reduction processes, hydrolase activity, transmembrane transport, and modulation of genes associated with fungal virulence. Pi-dependent modulation was observed mainly in genes encoding for transporter proteins or related to cell wall development, thereby advancing the current understanding regarding colonization and adaptation processes in response to challenging environments. We have also provided comprehensive evidence that suggests a role for PAC-3 as a global regulator in plant pathogenic fungi, thus presenting results that have the potential to be applied to various types of microbes, with diverse survival mechanisms. (AU)

• Processo FAPESP: 16/04274-6 - Fatores de transcrição e sinalização celular
• Beneficiário: Eriston Vieira Gomes
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/11319-1 - Fatores de transcrição e sinalização celular.
• Beneficiário: Maíra Pompeu Martins
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/03847-7 - Caracterização molecular de mecanismos envolvidos na patogenicidade e sinalização celular em fungos
• Beneficiário: Nilce Maria Martinez-Rossi
• Modalidade de apoio: Auxílio à Pesquisa - Temático