| Texto completo | |
| Autor(es): |
Giffoni de Carvalho, Jose Tarcisio
[1]
;
Schneider, Marion
[1]
;
Cuppari, Lilian
[1]
;
Grabulosa, Caren Cristina
[1]
;
Cendoroglo, Miguel
[2, 1]
;
Dalboni, Maria Aparecida
[1, 3]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Univ Fed Sao Paulo, UNIFESP, Div Nephrol, Sao Paulo, SP - Brazil
[2] Hosp Israelita Albert Einstein, Sao Paulo, SP - Brazil
[3] Univ Nove DeJulho UNINOVE, Postgrad Program Med, Sao Paulo, SP - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF RENAL NUTRITION; v. 29, n. 5, p. 454-461, SEP 2019. |
| Citações Web of Science: | 1 |
| Resumo | |
Background: End-stage renal disease results in B cell lymphopenia and low levels of vitamin D. Since the link between vitamin D deficiency and B lymphocytes dysfunction are not clear in patients with end-stage renal disease, we suggest that vitamin D adequacy and factors related to the homeostasis of these cells should be investigated. B lymphocytes homeostasis is a process mainly regulated signals of grown and death as interleukin (IL)-7, B cell-activating factor (BAFF)/BAFF-receptor and CD95 expression. Objective: As vitamin D serum levels were reduced in patients with end stage renal disease and it is associated with human B homeostasis, we evaluated the effect of cholecalciferol supplementation on dialysis. Design: Randomized, double blind clinical trial in dialysis patients with 250H Vitamin D deficiency for a period of 12 weeks. Main Outcome Measure: In a pilot study, we investigated the effect of cholecalciferol supplementation (100,000 UI once per week or placebo. In vitro, peripheral blood mononuclear cells isolated by Ficoll-Hypaque centrifugation from 12 healthy volunteers were incubated with healthy or uremic serum in the presence or absence of 25 (OH)DC with 5% CO. Results: There was an increase in the serum 25(OH)D level in the cholecalciferol group. No differences were found in BAFF and IL7 levels and CD95 and BAFF-R expression in B lymphocytes from patients on dialysis after cholecalciferol supplementation. Uremic serum induced an increase in the IL-7, BAFF, BAFF-R and CD95 expression compared with the control. However, we observed no effect of incubation of 25(OH)D3 and 1,25(OH)2D3 on the expression of IL-7, BAFF, BAFF-R and CD95 when incubated in the presence of normal or uremic serum. Conclusion: Our results suggest that vitamin D is not involved in mechanisms of regulation of differentiation and survival in B lymphocytes. In conclusion, further studies are needed to explore the effects of vitamin D on B lymphocytes to better evaluate the possible impact of vitamin D on humoral response in the CKD population. Published by Elsevier Inc. on behalf of the National Kidney Foundation, Inc. (AU) | |
| Processo FAPESP: | 12/21345-3 - Efeito do colecalciferol na modulação da expressão de toll-like receptors (TLR-7 e TLR-9) e síntese de IL-6 e INF-± em linfócitos B na uremia |
| Beneficiário: | Miguel Cendoroglo Neto |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 12/19314-2 - EFEITO DO COLECALCIFEROL NA MODULAÇÃO DA EXPRESSÃO DE TOLL-LIKE RECEPTORS (TLR-7 e TLR-9) E SÍNTESE DE IL-6 E INF-gamma EM LINFÓCITOS B NA UREMIA |
| Beneficiário: | José Tarcísio Giffoni de Carvalho Júnior |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |