Bacillus sp. isolated from banana waste and analysis of metabolic pathways in acidogenic systems in hydrogen production

Autor(es)

da Silva Mazareli, Raissa Cristina ^[1] ; Sakamoto, Isabel Kimiko ^[1] ; Silva, Edson Luiz ^[2] ; Amancio Varesche, Maria Bernadete ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Engn Sao Carlos, Dept Hydraul & Sanitat, Ave Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Chem Engn, Rod Washington Luiz, Km 235, SP 310, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Environmental Management; v. 247, p. 178-186, OCT 1 2019.
• Citações Web of Science: 0

Resumo

The autochthonous bacterial strain was isolated from banana waste (BW) for hydrogen production and organic acids from different pure substrates and BW. The potential production of H-2 and metabolic pathways were analyzed at pH 7.0 at 37 degrees C. Facultative anaerobic bacterium similar to Bacillus sp. was identified., with generation time (Tg) and growth rate (mu) of 0.43 h and 1.60 h(-)1(,) respectively, from glucose. The hydrogen production (P) using pure substrates was observed between 10.81 mmol.L-1 and 17.75 mmol.L-1 from xylose and maltose, respectively. The biggest and smallest hydrogen P of 18.77 mmol.L-1 and 1.72 mmol.L-1, were obtained with 3.5 and 0.5-1 of cellobiose, respectively. The highest hydrogen yield (126.93 ML.g(carbohydrates)(-1) (added)) was obtained with 2 5.L-1 cellobiose. In the assay using banana waste (5 g.L-1) the maximum P and yield (YH2) of 31.7 mmol.L-1 and 94.66 mL Hy g(carbohydrates added)(-1), was obtained respectively. The main metabolic pathway of hydrogen production by Bacillus sp. RM1 from banana waste was acetic-butyric acid of 487.69 g.L-1 and 535.88 g.L-1, respectively. The accessibility of various carbon sources by Bacillus sp. RM1 in fermentation can benefit the hydrogen production from complex organic substrates in the bioaugmentation process. (AU)

• Processo FAPESP: 16/20048-6 - Aplicação de bactérias celulolíticas e fermentativas isoladas a partir de resíduos de bananas (Musa spp.) na obtenção de compostos de interesse biotecnológico.
• Beneficiário: Raissa Cristina da Silva Mazareli
• Modalidade de apoio: Bolsas no Brasil - Doutorado