Silk fibroin films stabilizes and releases bioactive insulin for the treatment of corneal wounds

Autor(es)

Cubayachi, Camila ^[1] ; Lemos, Camila Nunes ^[1] ; Pereira, Francieli ^[1] ; Dias, Karina ^[1] ; Herculano, Rondinelli Donizetti ^[2] ; de Freitas, Osvaldo ^[1] ; Lopez, Renata F. V. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[2] Sao Paulo State Univ, Sch Pharmaceut Sci, Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: EUROPEAN POLYMER JOURNAL; v. 118, p. 502-513, SEP 2019.
• Citações Web of Science: 2

Resumo

Insulin (INS) is a protein that plays a crucial role in many cellular functions, including wound healing processes. However, its topical ocular administration is a challenge, mainly because of INS degradation and low retention time, which can significantly reduce its bioavailability. The objective of this work was to develop films based on silk fibroin (SF), a natural polymer with anti-inflammatory properties, containing INS aimed at the sustained release of a bioactive INS in the eye for the treatment of corneal wounds. SF films containing 100 IU/cm(2) INS and glycerin as plasticizer were prepared by casting. They were homogeneous, transparent, permeable to water vapor, with low swelling index and high mechanical resistance. Fourier transformed infrared spectroscopy and differential scanning calorimetry analyses suggested the beta-sheet/Silk II conformation of SF and the occurrence of non-covalent interactions between INS and SF. Scanning electron microscopy suggested that the INS was embedded inside the film in its native globular form. In fact, the INS released from the film maintained its native conformation, as observed by circular dichroism, in addition to conserving its biological activity in vivo, reducing the blood glycemia of Wistar rats. Sustained release, of approximately 1 IU/cm(2)/h(1/2) of INS per hour, following a burst release, suggests that the film could be effective in the recovery of injured corneal epithelium, avoiding the need for the various daily administrations. Therefore, SF films demonstrated potential to transport and release INS in a sustained manner, a promising strategy for the treatment of corneal wounds. (AU)

• Processo FAPESP: 14/22451-7 - Sistemas de liberação sustentada e direcionada de fármacos para o tecido epitelial
• Beneficiário: Renata Fonseca Vianna Lopez
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/19018-0 - Avaliação de formulações a base de fibroína da seda e insulina no tratamento tópico de feridas oculares
• Beneficiário: Camila Cubayachi
• Modalidade de apoio: Bolsas no Brasil - Doutorado