Doonan, Liam B.
Ibiji, Spike C.
Winter, Carlos E.
Morandini, Andre C.
Marques, Antonio C.
Long, Paul F.
Número total de Autores: 10
Afiliação do(s) autor(es):
 Kings Coll London, Fac Life Sci & Med, Sch Canc & Pharmaceut Sci, 150 Stamford St, London SE1 9NH - England
 Kings Coll London, Ctr Excellence Mass Spectrometry, 125 Coldharbour Lane, London SE5 9NU - England
 Univ Sao Paulo, Dept Parasitol, Inst Ciencias Biomed, Av Prof Lineu Prestes 1374, BR-05508900 Sao Paulo, SP - Brazil
 Univ Sao Paulo, Dept Microbiol, Inst Ciencias Biomed, Av Prof Lineu Prestes 1374, BR-05508900 Sao Paulo, SP - Brazil
 Univ Sao Paulo, Inst Biociencias, Dept Zool, Rua Matao, Trav 14, BR-05508090 Sao Paulo, SP - Brazil
 Univ Sao Paulo, Ctr Biol Marinha, Rodovia Manoel Hypolito do Rego, Km 131, 5, BR-11600000 Sao Sebastiao - Brazil
 Univ Sao Paulo, Fac Ciencias Farmaceut, Av Prof Lineu Prestes 580, B16, BR-05508000 Sao Paulo, SP - Brazil
Número total de Afiliações: 7
Tipo de documento:
AUG 1 2019.
Citações Web of Science:
In this quantitative proteomics study we determined the variety and relative abundance of toxins present in enriched preparations of two nematocyst types isolated from the primary tentacles of the adult medusa stage of the hydrozoan Olindias sambaquiensis. The two nematocyst types were microbasic mastigophores and microbasic euryteles, and these were recovered from the macerated tentacle tissues by using a differential centrifugation approach. Soluble protein extracts from these nematocysts were tagged with tandem mass tag isobaric labels and putative toxins identified using tandem mass spectrometry coupled with a stringent bioinformatics annotation pipeline. Astonishingly, the venom composition of the two capsule types was nearly identical, and there was also little difference in the comparative abundance of toxins between the two nematocyst preparations. This homogeneity suggested that the same toxin complement was present regardless of the penetrative ability of the nematocyst type. Predicted toxin protein families that constituted the venom closely matched those of the toxic proteome of O. sambaquiensis published four years previously, suggesting that venom composition in this species changes little over time. Retaining an array of different nematocyst types to deliver a single venom, rather than sustaining the high metabolic cost necessary to maintain a dynamically evolving venom, may be more advantageous, given the vastly different interspecific interactions that adult medusa encounter in coastal zones. (AU)