Venom Composition Does Not Vary Greatly Between Different Nematocyst Types Isolated from the Primary Tentacles of Olindias sambaquiensis (Cnidaria: Hydrozoa)

Autor(es)

Doonan, Liam B. ^[1] ; Lynham, Steven ^[2] ; Quinlan, Catherine ^[1] ; Ibiji, Spike C. ^[1] ; Winter, Carlos E. ^[3] ; Padilla, Gabriel ^[4] ; Jaimes-Becerra, Adrian ^[5] ; Morandini, Andre C. ^{[5, 6]} ; Marques, Antonio C. ^{[5, 6]} ; Long, Paul F. ^{[1, 7]}

Afiliação do(s) autor(es)

^[1] Kings Coll London, Fac Life Sci & Med, Sch Canc & Pharmaceut Sci, 150 Stamford St, London SE1 9NH - England

^[2] Kings Coll London, Ctr Excellence Mass Spectrometry, 125 Coldharbour Lane, London SE5 9NU - England

^[3] Univ Sao Paulo, Dept Parasitol, Inst Ciencias Biomed, Av Prof Lineu Prestes 1374, BR-05508900 Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Dept Microbiol, Inst Ciencias Biomed, Av Prof Lineu Prestes 1374, BR-05508900 Sao Paulo, SP - Brazil

^[5] Univ Sao Paulo, Inst Biociencias, Dept Zool, Rua Matao, Trav 14, BR-05508090 Sao Paulo, SP - Brazil

^[6] Univ Sao Paulo, Ctr Biol Marinha, Rodovia Manoel Hypolito do Rego, Km 131, 5, BR-11600000 Sao Sebastiao - Brazil

^[7] Univ Sao Paulo, Fac Ciencias Farmaceut, Av Prof Lineu Prestes 580, B16, BR-05508000 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOLOGICAL BULLETIN; v. 237, n. 1, p. 26-35, AUG 1 2019.
• Citações Web of Science: 1

Resumo

In this quantitative proteomics study we determined the variety and relative abundance of toxins present in enriched preparations of two nematocyst types isolated from the primary tentacles of the adult medusa stage of the hydrozoan Olindias sambaquiensis. The two nematocyst types were microbasic mastigophores and microbasic euryteles, and these were recovered from the macerated tentacle tissues by using a differential centrifugation approach. Soluble protein extracts from these nematocysts were tagged with tandem mass tag isobaric labels and putative toxins identified using tandem mass spectrometry coupled with a stringent bioinformatics annotation pipeline. Astonishingly, the venom composition of the two capsule types was nearly identical, and there was also little difference in the comparative abundance of toxins between the two nematocyst preparations. This homogeneity suggested that the same toxin complement was present regardless of the penetrative ability of the nematocyst type. Predicted toxin protein families that constituted the venom closely matched those of the toxic proteome of O. sambaquiensis published four years previously, suggesting that venom composition in this species changes little over time. Retaining an array of different nematocyst types to deliver a single venom, rather than sustaining the high metabolic cost necessary to maintain a dynamically evolving venom, may be more advantageous, given the vastly different interspecific interactions that adult medusa encounter in coastal zones. (AU)

• Processo FAPESP: 15/21007-9 - Reconhecendo a diversidade de águas-vivas (Medusozoa, Rhopaliophora)
• Beneficiário: André Carrara Morandini
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/52324-6 - Síntese e lacunas do conhecimento sobre os organismos da zona costeiro-marinha brasileira
• Beneficiário: Antonio Carlos Marques
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular
• Processo FAPESP: 10/50174-7 - Sistemática, ciclo de vida e padrões reprodutivos de medusas (Cnidaria: Medusozoa: Cubozoa e Scyphozoa) na Baixada Santista (São Paulo, Brasil): biodiversidade marinha
• Beneficiário: André Carrara Morandini
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular
• Processo FAPESP: 13/50484-4 - Biodiversity and distribution patterns of the Medusozoa form the South-Western Atlantic
• Beneficiário: Antonio Carlos Marques
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/50242-5 - Dimensões da vida marinha: padrões e processos de diversificação em cnidários planctônicos e bentônicos
• Beneficiário: Antonio Carlos Marques
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 10/06927-0 - Biodiversidade e áreas de endemismo de hidroides bentônicos (Cnidaria, Hydrozoa) da costa austral da América do Sul e Antártica
• Beneficiário: Thaís Pires Miranda
• Modalidade de apoio: Bolsas no Brasil - Doutorado