Venom Composition Does Not Vary Greatly Between Different Nematocyst Types Isolated from the Primary Tentacles of Olindias sambaquiensis (Cnidaria: Hydrozoa)

Author(s)

Doonan, Liam B. ^[1] ; Lynham, Steven ^[2] ; Quinlan, Catherine ^[1] ; Ibiji, Spike C. ^[1] ; Winter, Carlos E. ^[3] ; Padilla, Gabriel ^[4] ; Jaimes-Becerra, Adrian ^[5] ; Morandini, Andre C. ^{[5, 6]} ; Marques, Antonio C. ^{[5, 6]} ; Long, Paul F. ^{[1, 7]}

Affiliation

^[1] Kings Coll London, Fac Life Sci & Med, Sch Canc & Pharmaceut Sci, 150 Stamford St, London SE1 9NH - England

^[2] Kings Coll London, Ctr Excellence Mass Spectrometry, 125 Coldharbour Lane, London SE5 9NU - England

^[3] Univ Sao Paulo, Dept Parasitol, Inst Ciencias Biomed, Av Prof Lineu Prestes 1374, BR-05508900 Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Dept Microbiol, Inst Ciencias Biomed, Av Prof Lineu Prestes 1374, BR-05508900 Sao Paulo, SP - Brazil

^[5] Univ Sao Paulo, Inst Biociencias, Dept Zool, Rua Matao, Trav 14, BR-05508090 Sao Paulo, SP - Brazil

^[6] Univ Sao Paulo, Ctr Biol Marinha, Rodovia Manoel Hypolito do Rego, Km 131, 5, BR-11600000 Sao Sebastiao - Brazil

^[7] Univ Sao Paulo, Fac Ciencias Farmaceut, Av Prof Lineu Prestes 580, B16, BR-05508000 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: BIOLOGICAL BULLETIN; v. 237, n. 1, p. 26-35, AUG 1 2019.
• Web of Science Citations: 1

Abstract

In this quantitative proteomics study we determined the variety and relative abundance of toxins present in enriched preparations of two nematocyst types isolated from the primary tentacles of the adult medusa stage of the hydrozoan Olindias sambaquiensis. The two nematocyst types were microbasic mastigophores and microbasic euryteles, and these were recovered from the macerated tentacle tissues by using a differential centrifugation approach. Soluble protein extracts from these nematocysts were tagged with tandem mass tag isobaric labels and putative toxins identified using tandem mass spectrometry coupled with a stringent bioinformatics annotation pipeline. Astonishingly, the venom composition of the two capsule types was nearly identical, and there was also little difference in the comparative abundance of toxins between the two nematocyst preparations. This homogeneity suggested that the same toxin complement was present regardless of the penetrative ability of the nematocyst type. Predicted toxin protein families that constituted the venom closely matched those of the toxic proteome of O. sambaquiensis published four years previously, suggesting that venom composition in this species changes little over time. Retaining an array of different nematocyst types to deliver a single venom, rather than sustaining the high metabolic cost necessary to maintain a dynamically evolving venom, may be more advantageous, given the vastly different interspecific interactions that adult medusa encounter in coastal zones. (AU)

• FAPESP's process: 15/21007-9 - Recognizing the diversity of jellyfishes (Medusozoa, Rhopaliophora)
• Grantee: André Carrara Morandini
• Support Opportunities: Regular Research Grants
• FAPESP's process: 10/50174-7 - Systematics, life cycle and reproductive patterns of jellyfishes (Cnidaria: Medusozoa: Cubozoa and Scyphozoa) in the Baixada Santista (São Paulo, Brazil)
• Grantee: André Carrara Morandini
• Support Opportunities: BIOTA-FAPESP Program - Regular Research Grants
• FAPESP's process: 13/50484-4 - Biodiversity and distribution patterns of the Medusozoa form the South-Western Atlantic
• Grantee: Antonio Carlos Marques
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/50242-5 - Dimensions of marine life: patterns and process of diversifications in planktonic and benthic cnidarians
• Grantee: Antonio Carlos Marques
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
• FAPESP's process: 10/06927-0 - Biodiversity and areas of endemism of benthic hydroids (Cnidaria, Hydrozoa) from southern South America and Antarctica
• Grantee: Thaís Pires Miranda
• Support Opportunities: Scholarships in Brazil - Doctorate