Dissemination of bla(IMP-1)-carrying integron In86 among Klebsiella pneumoniae isolates harboring a new trimethoprim resistance gene dfr23

Autor(es)

Penteado, Andreia P. ^[1] ; Castanheira, Mariana ^[1] ; Pignatari, Antonio C. C. ^[1] ; Guimaraes, Thais ^[2] ; Mamizuka, Elsa M. ^[3] ; Gales, Ana C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Lab ALERTA, Div Infect Dis, BR-04039032 Sao Paulo - Brazil

^[2] Hosp Servidor Publ Estadual, Div Infect Dis, BR-04039901 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Dept Analises Clin, Fac Ciencias Farmaceut, BR-05508900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE; v. 63, n. 1, p. 87-91, JAN 2009.
• Citações Web of Science: 22

Resumo

The genetic context of the bla(IMP-1) gene was evaluated in 9 Klebsiella pneumoniae isolates recovered from 2 hospitals in Sao Paulo, Brazil. All isolates harbored a copy of In86 carrying bla(Imp-1), aac(6')-31, and aadAl. Eight strains from the same hospital also carried another class I integron harboring a new trimethoprim resistance gene (dfr23) that was chromosomally embedded. In86 was likely to be in a 30-kb nontransferable plasmid and was flanked upstream by a sequence identical to one identified in an IMP-1-producing Pseudomonas putida isolate. The bla(IMP-1)-carrying integron In86 was recently reported from nonfermentative bacilli isolated in Sao Paulo. These isolates appear to be the Source of this integron now acquired by K. pneumoniae strains from different hospitals in the same city. Metallo-beta-lactamase production is still rare among Enterobacteriaceae isolates in Brazil, but the acquisition of genetic structures carrying these mobile resistance determinants is worrisome and could lead to an increase in the prevalence of these phenotypes of resistance. (C) 2008 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 04/15339-4 - Caracterizacao dos mecanismos geneticos responsaveis pela disseminacao de determinantes de resistencia a carbapenens, blaimp-1 e blaspm-1, em amostras clinicas isoladas em hospitais brasileiros.
• Beneficiário: Ana Cristina Gales
• Modalidade de apoio: Auxílio à Pesquisa - Regular