Histopathological, histoenzymological, immunohistochemical and immunofluorescence analysis of tissue response to sealing materials after furcation perforation

Autor(es)

Silva, R. A. B. ^[1] ; Borges, A. T. N. ^{[1, 2]} ; Hernandez-Gaton, P. ^[3] ; de Queiroz, A. M. ^[1] ; Arzate, H. ^[4] ; Romualdo, P. C. ^[1] ; Nelson-Filho, P. ^[1] ; Silva, L. A. B. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Pediat Dent, Ave Cafe S-N, BR-14040904 Ribeirao Preto, SP - Brazil

^[2] Fac Amazonas, Manaus, Amazonas - Brazil

^[3] Univ Barcelona, Sch Dent, Dept Integrated Paediat Dent, Barcelona - Spain

^[4] Univ Nacl Autonoma Mexico, Fac Odontol, Div Estudios Posgrad & Invest, Lab Biol Periodontal, Mexico City, DF - Mexico

• Tipo de documento: Artigo Científico
• Fonte: International Endodontic Journal; v. 52, n. 10, p. 1489-1500, OCT 2019.
• Citações Web of Science: 0

Resumo

Aim: To evaluate in vivo tissue responses after sealing furcation perforations in dog's teeth with either Biodentine (TM), mineral trioxide aggregate (MTA) or gutta-percha, by means of histopathological, histoenzymological, immunohistochemical and immunofluorescence analysis. Methodology: After root canal treatment, perforations were created in the central region of the pulp chamber floor using a round diamond bur and filled with one or other of the materials. The animals were euthanized after 120 days, and the teeth (n = 30) were processed for histopathological analysis of new mineralized tissue formation and collagen fibre reinsertion, immunohistochemical analysis of osteopontin (OPN) and alkaline phosphatase (ALP) and immunofluorescence analysis for bone morphogenetic protein (BMP-2), cementum attachment protein (CAP), bone sialoprotein (BSP), osteocalcin (OCN) and cementum protein1 (CEMP1). Histoenzymology was performed for TRAP activity and osteoclast count. Data were analysed statistically (alpha = 0.05) using chi-square and Kruskal-Wallis tests. Results: Gutta-percha did not induce mineralized tissue formation. MTA and Biodentine(TM) formed mineralized tissue in 88% and 92% of specimens, respectively, with no significant difference (P > 0.05). Gutta-percha was associated with scattered collagen fibres parallel to the perforations. Groups treated with MTA or Biodentine(TM) had partial fibre reinsertion perpendicular to the newly formed mineralized tissue. All materials induced OPN and ALP expression, weakest for gutta-percha and strongest for MTA (P < 0.05). Only MTA induced BMP-2, BSP, OCN, CAP and CEMP1 expression. Osteoclast counts were similar in all groups (P = 0.97). Conclusions: Mineral trioxide aggregate and Biodentine(TM) were biocompatible, with formation of mineralized tissue and partial reinsertion of collagen fibres. In addition, the participation of several molecules by which calcium silicate-based materials induce the formation of mineralized tissue were noted, with expression of ALP and OPN mineralization markers, without interference in the number of osteoclasts. Only MTA stimulated the expression of proteins associated with the formation of a cementum-like mineralized tissue. (AU)

• Processo FAPESP: 14/25692-5 - Indução da diferenciação de células da polpa dentária e biomineralização: ensaios em cultura celular, em modelos animais e aplicação clínica em humanos
• Beneficiário: Léa Assed Bezerra da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular