Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Trans, trans-farnesol, an antimicrobial natural compound, improves glass ionomer cement properties

Texto completo
Autor(es):
Mostrar menos -
Freire de Castilho, Aline Rogeria [1] ; Rosalen, Pedro Luiz [2] ; de Souza Araujo, Isaac Jordao [3] ; Kitagawa, Igor Lebedenco [4] ; Goncalves de Araujo Costa, Cecilia Atem [5] ; Janal, Malvin N. [6] ; Alves, Marcelo Correa [7] ; Duarte, Simone [8] ; Lisboa Filho, Paulo Noronha [4] ; Stipp, Rafael Nobrega [9] ; Puppin-Rontani, Regina Maria [3, 1]
Número total de Autores: 11
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Pediat Dent, Piracicaba, SP - Brazil
[2] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, Piracicaba, SP - Brazil
[3] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Restorat Dent, Piracicaba, SP - Brazil
[4] State Univ Sao Paulo, Sch Sci, Dept Phys, Bauru, SP - Brazil
[5] Univ Fed Ceara, Dept Restorat Dent, Fortaleza, Ceara - Brazil
[6] NYU, Coll Dent, Dept Epidemiol & Hlth Promot, New York, NY - USA
[7] Univ Sao Paulo, Super Sch Agr Luiz De Queiroz, Tech Sect Informat Technol, Piracicaba, SP - Brazil
[8] Purdue Univ Indianapolis, Indiana Univ, Sch Dent, Dept Cariol Operat Dent & Dent Publ Hlth, Indianapolis, IN - USA
[9] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Oral Diag, Piracicaba, SP - Brazil
Número total de Afiliações: 9
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 14, n. 8 AUG 20 2019.
Citações Web of Science: 0
Resumo

A series of experiments were conducted to characterize a novel restorative material. We explored the effect on biological, physical and chemical properties of glass ionomer cement (GIC) adding-the naturally occurring tt-farnesol (900 mM). Two groups were accomplished for all assays: GIC+tt-farnesol and GIC (control). Biological assays: 1) agar diffusion against some cariogenic bacteria; 2) S. mutans biofilm formation and confocal laser scanning microscopy-CLSM. 3) gtfB, gtfC, gtfD, gbpB, vicR, and covR expression; 4) MTT and microscopic morphology. Physical properties assays: 1) roughness; 2) hardness; 3) compressive strength and 4) diametral tensile strength. Chemical assay: Raman spectroscopy. The adding of tt-farnesol to GIC led to larger zones of inhibition (p<0.05), biofilms with a short-term reduction in bacterial viability but similar biomass (p>0.05). Polysaccharides levels increased over time, similarly over groups (p>0.05). Viable and non-viable S. mutans were seen on the specimens' surface by CLSM but their virulence was not modulated by tt-farnesol. The tt-farnesol increased the HaCaT cell viability without impact on compressive and diametral tensile strength and roughness although the hardness was positively affected (p<0.05). Raman confirmed the presence of tt-farnesol. The incorporation of tt-farnesol into GIC inhibited the growth of cariogenic bacteria but had a little effect on the composition, structure and physiology of the biofilm matrices. Also, the tt-farnesol increased the hardness and the biocompatibility of the GIC, not influencing negatively other physical properties of the restorative material. (AU)

Processo FAPESP: 15/08871-6 - Efeito antibiofilme de um cimento de ionômero de vidro contendo produtos naturais
Beneficiário:Aline Rogéria Freire de Castilho
Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
Processo FAPESP: 14/01723-9 - Caracterização de um cimento de ionômero de vidro contendo produtos naturais: estudos in vitro e in vivo
Beneficiário:Aline Rogéria Freire de Castilho
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado