Trans, trans-farnesol, an antimicrobial natural compound, improves glass ionomer cement properties

Author(s):Show less -

Freire de Castilho, Aline Rogeria ^[1] ; Rosalen, Pedro Luiz ^[2] ; de Souza Araujo, Isaac Jordao ^[3] ; Kitagawa, Igor Lebedenco ^[4] ; Goncalves de Araujo Costa, Cecilia Atem ^[5] ; Janal, Malvin N. ^[6] ; Alves, Marcelo Correa ^[7] ; Duarte, Simone ^[8] ; Lisboa Filho, Paulo Noronha ^[4] ; Stipp, Rafael Nobrega ^[9] ; Puppin-Rontani, Regina Maria ^{[3, 1]}

Affiliation

^[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Pediat Dent, Piracicaba, SP - Brazil

^[2] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, Piracicaba, SP - Brazil

^[3] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Restorat Dent, Piracicaba, SP - Brazil

^[4] State Univ Sao Paulo, Sch Sci, Dept Phys, Bauru, SP - Brazil

^[5] Univ Fed Ceara, Dept Restorat Dent, Fortaleza, Ceara - Brazil

^[6] NYU, Coll Dent, Dept Epidemiol & Hlth Promot, New York, NY - USA

^[7] Univ Sao Paulo, Super Sch Agr Luiz De Queiroz, Tech Sect Informat Technol, Piracicaba, SP - Brazil

^[8] Purdue Univ Indianapolis, Indiana Univ, Sch Dent, Dept Cariol Operat Dent & Dent Publ Hlth, Indianapolis, IN - USA

^[9] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Oral Diag, Piracicaba, SP - Brazil

• Document type: Journal article
• Source: PLoS One; v. 14, n. 8 AUG 20 2019.
• Web of Science Citations: 0

Abstract

A series of experiments were conducted to characterize a novel restorative material. We explored the effect on biological, physical and chemical properties of glass ionomer cement (GIC) adding-the naturally occurring tt-farnesol (900 mM). Two groups were accomplished for all assays: GIC+tt-farnesol and GIC (control). Biological assays: 1) agar diffusion against some cariogenic bacteria; 2) S. mutans biofilm formation and confocal laser scanning microscopy-CLSM. 3) gtfB, gtfC, gtfD, gbpB, vicR, and covR expression; 4) MTT and microscopic morphology. Physical properties assays: 1) roughness; 2) hardness; 3) compressive strength and 4) diametral tensile strength. Chemical assay: Raman spectroscopy. The adding of tt-farnesol to GIC led to larger zones of inhibition (p<0.05), biofilms with a short-term reduction in bacterial viability but similar biomass (p>0.05). Polysaccharides levels increased over time, similarly over groups (p>0.05). Viable and non-viable S. mutans were seen on the specimens' surface by CLSM but their virulence was not modulated by tt-farnesol. The tt-farnesol increased the HaCaT cell viability without impact on compressive and diametral tensile strength and roughness although the hardness was positively affected (p<0.05). Raman confirmed the presence of tt-farnesol. The incorporation of tt-farnesol into GIC inhibited the growth of cariogenic bacteria but had a little effect on the composition, structure and physiology of the biofilm matrices. Also, the tt-farnesol increased the hardness and the biocompatibility of the GIC, not influencing negatively other physical properties of the restorative material. (AU)

• FAPESP's process: 15/08871-6 - Antibiofilm effect of a glass ionomer cement containing natural products
• Grantee: Aline Rogéria Freire de Castilho
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 14/01723-9 - Characterization of a glass ionomer cement containing natural products: in vitro and in vivo studies
• Grantee: Aline Rogéria Freire de Castilho
• Support Opportunities: Scholarships in Brazil - Post-Doctoral