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Effect of titanium dioxide nanotubes on biological properties of conventional glass ionomer cement

Abstract

Titanium dioxide nanotubes (n-TiO2) have been applied to dentistry to improve general properties of restorative materials. Our group has recently demonstrated that n-TiO2 may improve compression and wear resistance of conventional Glass Ionomer Cement (GIC), increase GIC hardness without affecting surface roughness, dentin micro shear-bond-strengh, and cell morphology as compared to GIC alone (Grant FAPESP #2016/13786-0). However, the ability of n-TiO2 to modulate bacteria and host response remains to be defined. Thus, the goals of the present study iare to: 1. define the impact of n-TiO2 on the antimicrobial properties of GIC; and 2. determine the potential of n-TiO2 to modulate the expression of immuno-inflammatory markers by fibroblasts (NIH/3T3 cells) treated or not with Fusobacterium nucleatum (Fn) total extract. In order to achieve those goals, n-TiO2 will be added at different concentrations (0%, 3%, 5%, 7% by weight) to GIC matrix (Ketac Molar EasyMix) and the following experiments performed: 1. Agar diffusion test (n=6): to determine a potential antimicrobial capacity of GIC, with or without, n-TiO2, bacteria inoculum (S. mutans or L. acidophilus) will be used, and chlorhexidine (0.12%) and distilled water will be used as positive and negative controls, respectively; 2. Cell viability (n=6): to assess the impact of GIC, with or without n-TiO2, on bacteria cell viability, 1x108 bacteria CFU/mL will be cultured in the presence of the tested materials and cell viability will be determined using the Live/Dead baclight fluorescence technique. Number of viable and non-viable cells will be obtained as the proportion of the total area of the disks in three random regions using the ImageJ program; 3. Cell morphology (n=6): the effect of tested materials on cell morphology will be defined by scanning electron microscopic analysis (SEM) (15 KV, 2000X), 4. Bacteria gene expression analysis (n=6): a potential impact of GIC, with or without n-TiO2, on transcript levels of vicR, covR, gtfB, gtfC, and egtfD in S. mutans and slpA, slpB e slpX for L. acidophilus will be determined by real-time quantitative PCR reactions; 5. Protein expression (n=6): NIH/3T3 cells will be cultured on GIC disks, with or without n-TiO2 (12 and 18 h), treated or not with Fn total extract (20, 2 and 0.2 ¼g/mL) and IL-1², IL-10, IL-6 and TNF secreted levels will be determined by mutiplex analysis using the MagPix system; and 6. Gene expression analysis in NIH/3T3 cells (n=6): IL-1², IL-10, IL-6, TNF transcript levels will be determined in NIH/3T3 cells cultured on GIC disks, with or without n-TiO2, treated or not with Fn total extract (3, 6 and 18 h). Experiments will be repeated, at least twice with three biological replicates, and in general, experimental times will be 1, 3 and 7 days (unless otherwise stated). Data were submitted to statistical analysis, considering the 5% level of significance (± = 0.05). (AU)

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Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ANA MARA DA SILVA MORAIS; YSADORA MAYARA REALI PEREIRA; ISAAC JORDÃO DE SOUZA-ARAÚJO; DANIEL FURTADO SILVA; VANESSA GALLEGO ARIAS PECORARI; ORISSON PONCE GOMES; FRANCISCO HUMBERTO NOCITI-JÚNIOR; REGINA MARIA PUPPIN-RONTANI; WALDEMIR FRANCISCO VIEIRA-JUNIOR; PAULO NORONHA LISBOA-FILHO; et al. TiO2 nanotube-containing glass ionomer cements display reduced aluminum release rates. Brazilian Oral Research, v. 36, . (19/14078-8, 16/13786-0)
DE SOUZA ARAUJO, ISAAC JORDAO; RICARDO, MARIANA GALLANTE; GOMES, ORISSON PONCE; GIOVANI, PRISCILA ALVES; PUPPIN-RONTANI, JULIA; PECORARI, VANESSA ARIAS; MARTINEZ, ELIZABETH FERREIRA; NAPIMOGA, MARCELO HENRIQUE; NOCITI JUNIOR, FRANCISCO HUMBERTO; PUPPIN-RONTANI, REGINA MARIA; et al. Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence. BRAZILIAN ORAL RESEARCH, v. 35, p. 12-pg., . (16/13786-0, 19/14078-8)
KANTOVITZ, KAMILA ROSAMILIA; CARLOS, NATALIA RUSSO; SILVA, IVANIA APARECIDA PIMENTA SANTOS; BRAIDO, CAROLINE; COSTA, BRUNA CAROLINA; KITAGAWA, IGOR LEBEDENCO; NOCITI-JR, FRANCISCO HUMBERTO; BASTING, ROBERTA TARKANY; DE FIGUEIREDO, FRANCINE KUHL PANZARELLA; LISBOA-FILHO, PAULO NORONHA. TiO2 nanotube-based nanotechnology applied to high-viscosity conventional glass-ionomer cement: ultrastructural analyses and physicochemical characterization. ODONTOLOGY, v. 111, n. 4, p. 13-pg., . (16/13786-0, 19/14078-8)
DA SILVA MORAIS, ANA MARA; REALI PEREIRA, YSADORA MAYARA; DE SOUZA-ARAUJO, ISAAC JORDAO; SILVA, DANIEL FURTADO; ARIAS PECORARI, VANESSA GALLEGO; GOMES, ORISSON PONCE; NOCITI-JUNIOR, FRANCISCO HUMBERTO; PUPPIN-RONTANI, REGINA MARIA; VIEIRA-JUNIOR, WALDEMIR FRANCISCO; LISBOA-FILHO, PAULO NORONHA; et al. TiO2 nanotube-containing glass ionomer cements display reduced aluminum release rates. BRAZILIAN ORAL RESEARCH, v. 36, p. 8-pg., . (16/13786-0, 19/14078-8)
ISAAC JORDÃO DE SOUZA ARAÚJO; MARIANA GALLANTE RICARDO; ORISSON PONCE GOMES; PRISCILA ALVES GIOVANI; JÚLIA PUPPIN-RONTANI; VANESSA ARIAS PECORARI; ELIZABETH FERREIRA MARTINEZ; MARCELO HENRIQUE NAPIMOGA; FRANCISCO HUMBERTO NOCITI JUNIOR; REGINA MARIA PUPPIN-RONTANI; et al. Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence. Brazilian Oral Research, v. 35, . (16/13786-0, 19/14078-8)

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