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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Cyclophosphamide Treatment Mimics Sub-Lethal Infections With Encephalitozoon intestinalis in Immunocompromised Individuals

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Autor(es):
Costa de Moura, Maria Lucia [1] ; Alvares-Saraiva, Anuska Marcelino [1] ; Perez, Elizabeth Cristina [1] ; Xavier, Jose Guilherme [1] ; Spadacci-Morena, Diva Denelle [2] ; Serantoni Moyses, Carla Renata [1] ; Dell'Armelina Rocha, Paulo Ricardo [1] ; Lallo, Maria Anete [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Paulista UNIP, Programa Posgrad Patol Ambiental & Expt, Sao Paulo - Brazil
[2] Inst Butantan, Dept Fisiopatol, Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: FRONTIERS IN MICROBIOLOGY; v. 10, SEP 25 2019.
Citações Web of Science: 0
Resumo

Microsporidia, including Encephalitozoon intestinalis, are emerging pathogens which cause opportunistic infections in immunocompromised patients, such as those with AIDS, cancer, the elderly and people on immunosuppressive drugs. Intestinal mucosa (IM) is crucial for developing an efficient adaptive immune response against pathogenic micro-organisms, thereby preventing their colonization and subsequent infection. As immunosuppressive drugs affect the intestinal immune response is little known. In the present study, we investigated the immune response to E. intestinalis infection in the IM and gut-associated lymphoid tissue (GALT) in cyclophosphamide (Cy) immunosuppressed mice, to mimic an immunocompromised condition. Histopathology revealed lymphoplasmacytic enteritis at 7 and 14 days-post-infection (dpi) in all infected groups, however, inflammation diminished at 21 and 28 dpi. Cy treatment also led to a higher number of E. intestinalis spores and lesions, which reduced at 28 dpi. In addition, flow cytometry analysis demonstrated CD4(+) and CD8(+) T cells to be predominant immune cells, with up-regulation in both Th1 and Th2 cytokines at 7 and 14 dpi, as demonstrated by histopathology. In conclusion, Cy treatment reduced GALT (Peyer's plaques and mesenteric lymph nodes) and peritoneum populations but increased the T-cell population in the intestinal mucosa and the production of pro-and anti-inflammatory cytokines, which were able to eliminate this opportunistic fungus and reduced the E. intestinalis infection. (AU)

Processo FAPESP: 15/25948-2 - Microsporidiose murina - o papel de Macrófagos M1 e M2, fagócitos derivados de Células B-1 e eferocitose
Beneficiário:Maria Anete Lallo
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 12/51727-5 - O papel das células B-1 na infecção experimental pelo Encephalitozoon cuniculi em camundongos
Beneficiário:Maria Anete Lallo
Linha de fomento: Auxílio à Pesquisa - Regular