| Texto completo | |
| Autor(es): |
Kronka Sosthenes, Marcia Consentino
[1, 2, 3]
;
Diniz, Daniel Guerreiro
[2]
;
Roodselaar, Jay
[3]
;
Abadie-Guedes, Ricardo
[4]
;
Chaves de Siqueira Mendes, Fabiola de Carvalho
[5, 2]
;
Fernandes, Taiany Nogueira
[2]
;
Bittencourt, Jackson Cioni
[6, 1]
;
Picanco Diniz, Cristovam Wanderley
[2]
;
Anthony, Daniel Clive
[3]
;
Araujo Guedes, Rubem Carlos
[4]
Número total de Autores: 10
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Dept Anat, Lab Neuroanat Quim, Sao Paulo - Brazil
[2] Univ Fed Para, Hosp Univ Joao de Barros Barreto, Inst Ciencias Biol, Lab Invest Neurodegeneracao & Infecaao, Belem, Para - Brazil
[3] Univ Oxford, Dept Pharmacol, Lab Expt Neuropathol, Oxford - England
[4] Univ Fed Pernambuco, Dept Nutr, Lab Fisiol Nutr Naide Teodasio, Recife, PE - Brazil
[5] Ctr Univ Estado Para, Curso Med, Belem, Para - Brazil
[6] Univ Sao Paulo, Inst Psicol, Nucleo Neurociencias & Comportamento, Sao Paulo - Brazil
Número total de Afiliações: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | FRONTIERS IN NEUROSCIENCE; v. 13, SEP 25 2019. |
| Citações Web of Science: | 0 |
| Resumo | |
Early growth response-1 (Egr-1), defined as a zinc finger transcription factor, is an upstream master switch of the inflammatory response, and its expression can be used to investigate the spatial and temporal extent of inflammatory changes in the brain. Cortical spreading depression (CSD) is characterized as a slowly propagating (2-5 mm/min) depolarization wave through neurons and astrocytes in humans that contributes to migraines and possibly to other brain pathologies. In rodents, CSD can be induced experimentally, which involves unilateral depolarization that is associated with microglial and astrocyte responses. The impact of CSD on structures beyond the affected hemisphere has not been explored. Here, we used an optical fractionator method to investigate potential correlations between the number of and period of the eletrophysiologic record of CSD phenomena and Egr-1 expression in ipsilateral and contralateral hemispheres. CSD was elicited by the restricted application of a 2% KCl solution over the left premotor cortex. Electrophysiological events were recorded using a pair of Ag/AgCl agar-Ringer electrodes for 2 or 6 h. An optical fractionator was applied to count the Egr-1 positive cells. We found that CSD increased Egr-1 expression in a time- and event-dependent manner in the ipsilateral/left hemisphere. Although CSD did not cross the midline, multiple CSD inductions were associated with an increased number of Egr-1 positive cells in the contralateral/right hemisphere. Thus, repeated CSD waves may have far reaching effects that are more global than previously considered possible. The mechanism of contralateral expression is unknown, but we speculate that callosal projections from the depolarized hemisphere may be related to this phenomenon. (AU) | |
| Processo FAPESP: | 10/52068-0 - A participação do hormônio concentrador de melanina no controle da lactação |
| Beneficiário: | Jackson Cioni Bittencourt |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 16/02224-1 - A existência da neurogênese durante o período da lactação |
| Beneficiário: | Jackson Cioni Bittencourt |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |