DDAH1 and DDAH2 polymorphisms associate with asymmetrical dimethylarginine plasma levels in erectile dysfunction patients but not in healthy controls

Autor(es):Mostrar menos -

Brites-Anselmi, Guilhermo ^[1] ; Milanez Azevedo, Ana Maria ^[2] ; Lima Miyazaki, Anderson Heiji ^[1] ; Pinheiro, Lucas Cezar ^[1] ; Coeli-Lacchin, Fernanda Borchers ^[3] ; de Andrade, Murilo Ferreira ^[4] ; Fernandes Molina, Carlos Augusto ^[4] ; Tucci, Jr., Silvio ^[4] ; Hirsch, Emilio ^[5] ; Tanus-Santos, Jose Eduardo ^[2] ; Lacchini, Riccardo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Coll Nursing, Dept Psychiat Nursing & Human Sci, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pharmacol, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Analyses Toxicol & Food Sci, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Surg & Anat, Sao Paulo - Brazil

^[5] Univ Torino, Dept Mol Biotechnol & Hlth Sci, Turin - Italy

• Tipo de documento: Artigo Científico
• Fonte: NITRIC OXIDE-BIOLOGY AND CHEMISTRY; v. 92, p. 11-17, NOV 1 2019.
• Citações Web of Science: 0

Resumo

Erectile Dysfunction (ED) is one of the main complaints of aging male. A reduced production of Nitric Oxide (NO) may be involved in ED pathogenesis. NO is synthesized from L-Arginine, and asymmetrical dimethylarginine inhibits all NO synthases. DDAH1 and DDAH2 are genes that encode enzymes responsible for metabolizing ADMA. We aimed to assess whether: 1) ADMA and nitrite levels associated with ED risk and with symptoms intensity; and whether 2) DDAH1 and DDAH2 gene polymorphisms associate with changes in biochemical data, and with ED risk and symptoms intensity. In this study were included 98 healthy controls and 130 ED patients. ADMA levels were measured by ELISA and nitrite levels by Chemiluminescence. DDAH1 and DDAH2 polymorphisms were assessed by Taqman assays. We found that ED had increased nitrite levels and lower ADMA levels than Control group (P < 0.05). We found a significant correlation of ADMA with Nitrite levels only in ED (B = -0.57, P < 0.001). Genotypes and haplotypes of DDAH1 were associated with ADMA levels in ED (P < 0.05), while haplotypes of DDAH2 were associated with levels of nitrite in ED (P < 0.05). Erectile dysfunction patients show an association between DDAH1 and DDAH2 polymorphisms with ADMA levels, which in turn are negatively correlated with nitrite levels. This is not evident on healthy controls. (AU)

• Processo FAPESP: 16/04449-0 - Bases genéticas da disfunção erétil: dimetil arginina assimétrica e genes relacionados afetando a resposta ao sildenafil
• Beneficiário: Riccardo Lacchini
• Modalidade de apoio: Auxílio à Pesquisa - Regular