Cytotaxonomy of Trypanosoma cruzi (Chagas, 1909): Differentiation of T. cruzi I (TcI) and T. cruzi II (TcII) Genotypes Using Cytogenetic Markers

Autor(es)

Bortolozo de Oliveira, Ana Beatriz ^[1] ; Ribeiro, Aline Rimoldi ^[2] ; Madeira, Fernanda Fernandez ^[1] ; Cesaretto, Natalia Regina ^[3] ; da Rosa, Joao Aristeu ^[4] ; Vilela de Azeredo-Oliveira, Maria Tercilia ^[1] ; Chaboli Alevi, Kaio Cesar ^{[4, 3]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, IBILCE UNESP, Lab Biol Celular, Dept Biol, Inst Biociencias Letras & Ciencias Exat, Sao Jose Do Rio Preto - Brazil

^[2] Univ Estadual Campinas, Inst Biol, Dept Parasitol, UNICAMP Ave Bertrand Russel, Campinas, SP - Brazil

^[3] Univ Estadual Paulista, Inst Biociencias Botucatu, IBB UNESP, Botucatu, SP - Brazil

^[4] Univ Estadual Paulista, FCFAR UNESP, Fac Ciencias Farmaceut, Lab Parasitol, Dept Ciencias Biol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: American Journal of Tropical Medicine and Hygiene; v. 101, n. 3, p. 605-607, 2019.
• Citações Web of Science: 0

Resumo

Chagas disease is a public health problem caused by the Trypanosoma cruzi, and the T. cruzi I (TcI) and T. cruzi II (TcII) groups are considered important genotypes from the clinical point of view. Currently, the groups need to be molecularly analyzed for their identification; thus, we cytogenetically analyzed these groups with the objective of developing more accessible techniques for the characterization of these parasites. TcI and TcII groups were differentiated by nucleus characterization with lacto-acetic orcein (TcI-nucleus with positive heteropycnosis and TcII-nucleus with negative heteropycnosis), emphasizing the importance of the application of this technique for epidemiological and clinical studies of Chagas disease. (AU)

• Processo FAPESP: 17/05015-7 - Estudo sobre hibridização em Triatoma sordida (Stal, 1859) (Hemiptera, Reduviidae): dinâmica evolutiva, viabilidade reprodutiva, caracterização morfo-molecular e suas implicações taxonômicas
• Beneficiário: Kaio Cesar Chaboli Alevi
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado