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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Reflex arc recovery after spinal cord dorsal root repair with platelet rich plasma (PRP)

Texto completo
Autor(es):
de Castro, Mateus Vidigal [1] ; Ramos da Silva, Monize Valeria [1] ; Chiarotto, Gabriela Bortolanca [2] ; Volpe, Bruno Bosh [3] ; Santana, Maria Helena [4] ; Malheiros Luzo, Angela Cristina [5] ; Rodrigues de Oliveira, Alexandre Leite [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Inst Biol, Dept Struct & Funct Biol, Campinas, SP - Brazil
[2] Univ Ctr Herminio Ometto Fdn, Campinas, SP - Brazil
[3] Reg Ctr Univ Espirito Santo Pinhal, Sao Paulo - Brazil
[4] Univ Estadual Campinas, Sch Chem Engn, Dept Engn Mat & Bioproc, Campinas, SP - Brazil
[5] Univ Estadual Campinas, Umbil Cord Blood Bank, Haematol & Hemotherapy Ctr, Campinas, SP - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: Brain Research Bulletin; v. 152, p. 212-224, OCT 2019.
Citações Web of Science: 0
Resumo

Spinal dorsal roots can be affected by a wide range of lesions, leading to a significant loss of proprioceptive information transmission and greatly affecting motor behavior. In this context, the reimplantation of lesioned roots with platelet-rich plasma (PRP) may allow nerve regeneration. Therefore, the present study evaluated sensorimotor improvement following dorsal root rhizotomy and repair with PRP. For this purpose, female Lewis rats were subjected to unilateral rhizotomy (RZ) of the L4-L6 dorsal roots and divided into the following groups: (1) the unlesioned control group; (2) the group that underwent rhizotomy (RZ) without repair; and (3) the group that underwent RZ followed by root repair with PRP. PRP was obtained from human blood and characterized regarding platelet concentration, integrity, and viability. Reflex arc recovery was evaluated weekly for eight weeks by the electronic von Frey method. The spinal cords were processed 1 week postlesion to evaluate the in vivo gene expression of TNFa, TGF-beta, BDNF, GDNF, VEGF, NGF, IL-4, IL-6, IL-13 by qRT-PCR and eight weeks postlesion to evaluate changes in the glial response (GFAP and Iba-1) and excitatory synaptic circuits (VGLUT1) by immunofluorescence. The results indicated that PRP therapy partially restores the paw withdrawal reflex over time, indicating the reentry of primary afferents from the dorsal root ganglia into the spinal cord without exacerbating glial reactivity. Additionally, the analysis of mRNA levels showed that PRP therapy has immunomodulatory properties. Overall, the present data suggest that the repair of dorsal roots with PRP may be considered a promising approach to improve sensorimotor recovery following dorsal rhizotomy. (AU)

Processo FAPESP: 15/26206-0 - Recuperação sensório-motora após transecção e reparo de raízes dorsais medulares com plasma rico em plaquetas (PRP) e tratamento com células tronco embrionárias humanas (hESC).
Beneficiário:Mateus Vidigal de Castro
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 18/05006-0 - Recuperação sensório-motora após axotomia de raízes medulares: emprego de diferentes abordagens experimentais
Beneficiário:Alexandre Leite Rodrigues de Oliveira
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 14/06892-3 - Utilização de células tronco mesenquimais na interface do sistema nervoso central e periférico: reparo de lesões proximais
Beneficiário:Alexandre Leite Rodrigues de Oliveira
Linha de fomento: Auxílio à Pesquisa - Temático