A proteomics outlook towards the elucidation of epithelial-mesenchymal transition molecular events

Autor(es)

Silvestrini, Virginia Campos ^{[1, 2]} ; Lanfredi, Guilherme Pauperio ^[1] ; Masson, Ana Paula ^[1] ; Poersch, Aline ^[1] ; Ferreira, Germano Aguiar ^[2] ; Thome, Carolina Hassibe ^[2] ; Faca, Vitor Marcel ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Biochem & Immunol, FMRP, Av Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Hemotherapy Ctr Ribeirao Preto, Ctr Cell Based Therapy, Rua Tenente Catao Roxo 2501, BR-14051140 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: MOLECULAR OMICS; v. 15, n. 5, p. 316-330, OCT 1 2019.
• Citações Web of Science: 0

Resumo

The main cause of death in cancer is the spread, or metastasis, of cancer cells to distant organs with consequent tumor formation. Additionally, metastasis is a process that demands special attention, as the cellular transformations make cancer at this stage very difficult or occasionally even impossible to be cured. The main process that converts epithelial tumor cells to mesenchymal-like metastatic cells is the Epithelial to Mesenchymal Transition (EMT). This process allows stationary and polarized epithelial cells, which are connected laterally to several types of junctions as well as the basement membrane, to undergo multiple biochemical changes that enable disruption of cell-cell adherence and apical-basal polarity. Moreover, the cells undergo important reprogramming to remodel the cytoskeleton and acquire mesenchymal characteristics such as enhanced migratory capacity, invasiveness, elevated resistance to apoptosis and a large increase in the production of ECM components. As expected, the alterations of the protein complement are extensive and complex, and thus exploring this by proteomic approaches is of particular interest. Here we review the overall findings of proteome modifications during EMT, mainly focusing on molecular signatures observed in multiple proteomic studies as well as coordinated pathways, cellular processes and their clinical relevance for altered proteins. As a result, an interesting set of proteins is highlighted as potential targets to be further investigated in the context of EMT, metastasis and cancer progression. (AU)

• Processo FAPESP: 17/03960-6 - Caracterização do papel das enzimas envolvidas em modificações pós-traducionais que modulam a estabilidade e translocação proteica durante a Transição Epitélial-Mesenquimal
• Beneficiário: Virgínia Campos Silvestrini
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 16/03809-3 - Estudo proteômico dirigido da localização e translocação subcelular de proteínas na transição epitélio-mesenquimal
• Beneficiário: Vitor Marcel Faça
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/08135-2 - CTC - Centro de Terapia Celular
• Beneficiário: Dimas Tadeu Covas
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs