Study of mastoparan analog peptides against Candida albicans and safety in zebrafish embryos (Danio rerio)

Autor(es)

Galeane, Mariana C. ^[1] ; Gomes, Paulo C. ^[1] ; Singulani, Junya de L. ^[1] ; de Souza, Bibiana M. ^[2] ; Palma, Mario S. ^[2] ; Mendes-Giannini, Maria J. S. ^[1] ; Almeida, Ana M. F. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Clin Anal, BR-14800903 Araraquara, SP - Brazil

^[2] Sao Paulo State Univ UNESP, CEIS LSBZ, Dept Biol, Inst Biosci, BR-13506900 Rio Claro, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FUTURE MICROBIOLOGY; v. 14, n. 13, p. 1087-1097, SEP 2019.
• Citações Web of Science: 0

Resumo

Aim: In this work, mastoparan analog peptides from wasp venom were tested against Candida albicans and safety assays were performed using cell culture and model zebrafish. Materials \& methods: Minimal inhibitory concentration was determined and toxicity was performed using human skin keratinocyte and embryo zebrafish. Also, permeation of peptides through embryo chorion was performed. Results: The peptides demonstrated anti-C. albicans activity, with low cytotoxicity and nonteratogenicity in Danio rerio. The compounds had different permeation through chorion, suggesting that this occurs due to modifications in their amino acid sequence. Conclusion: The results showed that the studied peptides can be used as structural study models for novel potential antifungal agents. (AU)

• Processo FAPESP: 17/06658-9 - Plataforma para desenvolvimento de antifúngicos em sistema nanoestruturado lipídico visando eficácia e segurança em modelos animais alternativos
• Beneficiário: Junya de Lacorte Singulani
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/11836-0 - SELEÇÃO DE ALVOS TERAPÊUTICOS E/OU BIOMARCADORES A PARTIR DA RESPOSTA TRANSCRICIONAL GLOBAL DA INFECÇÃO DE MACRÓFAGOS ALVEOLARES POR BIOFILMES DE H. capsulatum
• Beneficiário: Ana Marisa Fusco Almeida
• Modalidade de apoio: Auxílio à Pesquisa - Regular