Study of mastoparan analog peptides against Candida albicans and safety in zebrafish embryos (Danio rerio)

Author(s)

Galeane, Mariana C. ^[1] ; Gomes, Paulo C. ^[1] ; Singulani, Junya de L. ^[1] ; de Souza, Bibiana M. ^[2] ; Palma, Mario S. ^[2] ; Mendes-Giannini, Maria J. S. ^[1] ; Almeida, Ana M. F. ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Clin Anal, BR-14800903 Araraquara, SP - Brazil

^[2] Sao Paulo State Univ UNESP, CEIS LSBZ, Dept Biol, Inst Biosci, BR-13506900 Rio Claro, SP - Brazil

• Document type: Journal article
• Source: FUTURE MICROBIOLOGY; v. 14, n. 13, p. 1087-1097, SEP 2019.
• Web of Science Citations: 0

Abstract

Aim: In this work, mastoparan analog peptides from wasp venom were tested against Candida albicans and safety assays were performed using cell culture and model zebrafish. Materials \& methods: Minimal inhibitory concentration was determined and toxicity was performed using human skin keratinocyte and embryo zebrafish. Also, permeation of peptides through embryo chorion was performed. Results: The peptides demonstrated anti-C. albicans activity, with low cytotoxicity and nonteratogenicity in Danio rerio. The compounds had different permeation through chorion, suggesting that this occurs due to modifications in their amino acid sequence. Conclusion: The results showed that the studied peptides can be used as structural study models for novel potential antifungal agents. (AU)

• FAPESP's process: 17/06658-9 - Platform for antifungal development in a nanostructured lipid system aiming at efficacy and safety in alternative animal models
• Grantee: Junya de Lacorte Singulani
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 16/11836-0 - SELECTION OF THERAPEUTIC TARGETS AND/OR BIOMARKERS FROM GLOBAL RESPONSE TRANSCRIPTIONAL OF ALVEOLAR MACROPHAGES INFECTED BY H. capsulatum BIOFILMS
• Grantee: Ana Marisa Fusco Almeida
• Support Opportunities: Regular Research Grants