| Texto completo | |
| Autor(es): |
Marzuca-Nassr, G. N.
[1, 2]
;
Fortes, M. A. S.
[1]
;
Guimaraes-Ferreira, L.
[3]
;
Murata, G. M.
[1]
;
Vitzel, K. F.
[1, 4]
;
Vasconcelos, D. A. A.
[1]
;
Bassit, R. A.
[5]
;
Curi, R.
[1, 6]
Número total de Autores: 8
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Fisiol & Biofis, Sao Paulo, SP - Brazil
[2] Univ La Frontera, Dept Internal Med, Fac Med, Temuco - Chile
[3] Univ Fed Espirito Santo, Ctr Educ Fis & Desportos, Grp Estudos Fisiol Muscular & Performance Humana, Vitoria, ES - Brazil
[4] Massey Univ, Sch Hlth Sci, Coll Hlth, Auckland - New Zealand
[5] Univ Sao Paulo, Inst Ciencias Biomed, Dept Biol Celular & Desenvolvimento, Sao Paulo, SP - Brazil
[6] Univ Cruzeiro Sul, Programa Posgrad Interdisciplinar Ciencias Saude, Sao Paulo, SP - Brazil
Número total de Afiliações: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Brazilian Journal of Medical and Biological Research; v. 52, n. 10 2019. |
| Citações Web of Science: | 0 |
| Resumo | |
The effect of a short-term creatine supplementation on hindlimb suspension (HS)-induced muscle atrophy was investigated. Creatine monohydrate (5 g/kg b.w. per day) or placebo, divided in 2 daily doses, was given by oral gavage for 5 days. Rats were maintained in HS with dietary supplementation concomitantly for 5 days. Body weight, soleus and EDL muscle masses, and cross-sectional areas (CSA) of the muscle fibers were measured. Signaling pathways associated with skeletal muscle mass regulation (FST, MSTN, FAK, IGF-1, MGF, Akt, mTOR, atrogin-1, and MuRF1 expressions, and Akt, S6, GSK3B, and 4EBP1 proteins) were evaluated in the muscles. Soleus muscle exhibited more atrophy than the EDL muscle due to HS. Creatine supplementation attenuated the decrease of wet weight and increased p-4EBP1 protein in the EDL muscle of HS rats. Also, creatine increased mTOR and atrogin-1 expressions in the same muscle and condition. In the absence of HS, creatine supplementation increased FAK and decreased MGF expressions in the EDL muscle. Creatine attenuated the increase in FST expression due to HS in the soleus muscle. MuRF1 expression increased in the soleus muscle due to creatine supplementation in HS animals whereas atrogin-1 expression increased still further in this group compared with untreated HS rats. In conclusion, short-term creatine supplementation changed protein metabolism signaling in soleus and EDL muscles. However, creatine supplementation only slightly attenuated the mass loss of both muscles and did not prevent the CSA reduction and muscle strength decrease induced by HS for 5 days. (AU) | |
| Processo FAPESP: | 13/17734-7 - Efeito do treinamento físico aeróbio sobre o infiltrante inflamatório no tumor de pacientes caquéticos e não caquéticos. |
| Beneficiário: | Reinaldo Abunasser Bassit |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |