Optimization of limonene biotransformation for the production of bulk amounts of alpha-terpineol

Autor(es)

Molina, Gustavo ^{[1, 2, 3]} ; Pessoa, Marina G. ^[1] ; Bicas, Juliano L. ^[1] ; Fontanille, Pierre ^{[2, 4]} ; Larroche, Christian ^{[2, 4]} ; Pastore, Glaucia M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Food Engn, Lab Bioflavors, Dept Food Sci, Campinas, SP - Brazil

^[2] Univ Clermont Auvergne, Inst Pascal, TSA 60026, F-63178 Aubiere - France

^[3] Inst Sci & Technol UFVJM, Lab Food Biotechnol, Food Engn, Diamantina, MG - Brazil

^[4] CNRS, IP, UMR 6602, F-63178 Aubiere - France

• Tipo de documento: Artigo Científico
• Fonte: Bioresource Technology; v. 294, DEC 2019.
• Citações Web of Science: 3

Resumo

The biotransformation of R-(+)-limonene into high concentrations of R-(+)-alpha-terpineol by Sphingobium sp. was investigated in order to optimize the main process variables (pH, biocatalyst concentration, substrate concentration, temperature and agitation). This strategy comprised the screening of variables by a Plackett-Burman design followed by a Central Composite Design. The statistical analysis showed that the optimal alpha-terpineol production were at 28 degrees C and pH 7.0, with a limonene concentration of 350 g/L of organic phase agitation of 200 rpm and a biocatalyst concentration of 2.8 g/L of aqueous phase (OD600 = 8). Further trials showed that the R-(+)-alpha-terpineol concentration was higher (240 g/L after 96 h) when using a ratio of 1:3 (v.v(-1)) of organic:aqueous phases. However, the total production and yield (in terms of biomass) of alpha-terpineol would be maximized for an aqueous:organic ratio of 1:1. The experimental design optimization adopted herein was an effective tool for this type of study. (AU)

• Processo FAPESP: 16/21619-7 - Avaliação das propriedades biológicas de produtos da biotransformação do limoneno
• Beneficiário: Juliano Lemos Bicas
• Modalidade de apoio: Auxílio à Pesquisa - Regular