| Full text | |
| Author(s): |
Molina, Gustavo
[1, 2, 3]
;
Pessoa, Marina G.
[1]
;
Bicas, Juliano L.
[1]
;
Fontanille, Pierre
[2, 4]
;
Larroche, Christian
[2, 4]
;
Pastore, Glaucia M.
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Estadual Campinas, Sch Food Engn, Lab Bioflavors, Dept Food Sci, Campinas, SP - Brazil
[2] Univ Clermont Auvergne, Inst Pascal, TSA 60026, F-63178 Aubiere - France
[3] Inst Sci & Technol UFVJM, Lab Food Biotechnol, Food Engn, Diamantina, MG - Brazil
[4] CNRS, IP, UMR 6602, F-63178 Aubiere - France
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Bioresource Technology; v. 294, DEC 2019. |
| Web of Science Citations: | 3 |
| Abstract | |
The biotransformation of R-(+)-limonene into high concentrations of R-(+)-alpha-terpineol by Sphingobium sp. was investigated in order to optimize the main process variables (pH, biocatalyst concentration, substrate concentration, temperature and agitation). This strategy comprised the screening of variables by a Plackett-Burman design followed by a Central Composite Design. The statistical analysis showed that the optimal alpha-terpineol production were at 28 degrees C and pH 7.0, with a limonene concentration of 350 g/L of organic phase agitation of 200 rpm and a biocatalyst concentration of 2.8 g/L of aqueous phase (OD600 = 8). Further trials showed that the R-(+)-alpha-terpineol concentration was higher (240 g/L after 96 h) when using a ratio of 1:3 (v.v(-1)) of organic:aqueous phases. However, the total production and yield (in terms of biomass) of alpha-terpineol would be maximized for an aqueous:organic ratio of 1:1. The experimental design optimization adopted herein was an effective tool for this type of study. (AU) | |
| FAPESP's process: | 16/21619-7 - Evaluation of the biological properties of limonene biotransformation products |
| Grantee: | Juliano Lemos Bicas |
| Support Opportunities: | Regular Research Grants |