Development of plasmonic ELISA for the detection of anti-Leishmania sp. IgG antibodies

Autor(es)

dos Santos Maciel, Marilene Oliveira ^[1] ; Soares, Matheus Fujimura ^[1] ; Costa, Sidnei Ferro ^[1] ; Bragato, Jaqueline Poleto ^[1] ; de Freitas, Jessica Henrique ^[1] ; Venturin, Gabriela Lovizutto ^[1] ; Melo, Larissa Martins ^[1] ; Rebech, Gabriela Torres ^[1] ; Reed, Steve ^[2] ; Felix de Lima, Valeria Marcal ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Sch Vet Med Cine, Dept Clin Surg & Anim Reprod, Aracatuba, SP - Brazil

^[2] Infect Dis Res Inst, Seattle, WA - USA

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF IMMUNOLOGICAL METHODS; v. 474, NOV 2019.
• Citações Web of Science: 1

Resumo

Recently, a novel Enzyme-Linked Immunosorbent Assay (ELISA) strategy has emerged, known as ``plasmonic ELISA{''} (pELISA), which enables the detection of disease biomarkers at low concentrations with the naked eye. For the first time, this research has developed a signal-generation mechanism for the detection of anti-Leishmania sp. IgG antibodies with the naked eye using pELISA. The immunoassay incorporates an indirect ELISA with successive growth of gold nanoparticles to obtain blue or red-colored solutions in the presence or absence of anti-Leishmania sp. IgG antibodies in canine serum, respectively. The technique we developed was successfully tested in canine serum positive and negative for canine leishmaniasis (CanL), and was shown to be an effective method that could be used as an additional tool for CanL diagnosis. It will be particularly useful in resource-constrained countries, because it does not require sophisticated instruments to read the results, increasing the practicality of CanL detection in these areas. (AU)

• Processo FAPESP: 17/11016-6 - ELISA plasmônica na detecção ultra-sensível de anticorpos IgG anti-Leishmania sp
• Beneficiário: Marilene Oliveira dos Santos Maciel
• Modalidade de apoio: Bolsas no Brasil - Mestrado