A simple and fast method for determination of benzocaine and lidocaine in pharmaceutical formulations by capillary electrophoresis with spectrophotometric detection

Autor(es)

Junger, Ayandra Santos ^[1] ; Souza de Jesus, Francielly Fernanda ^[1] ; Fracassi da Silva, Jose Alberto ^{[1, 2]} ; Daniel, Daniela ^[3] ; de Jesus, Dosil Pereira ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, UNICAMP, Inst Chem, Campinas, SP - Brazil

^[2] Inst Nacl Ciencia & Tecnol Bioanalit, Campinas, SP - Brazil

^[3] Agilent Technol, Barueri, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SEPARATION SCIENCE PLUS; v. 2, n. 11, p. 422-427, NOV 2019.
• Citações Web of Science: 0

Resumo

A novel, simple, and rapid method for the determination of the anesthetics benzocaine and lidocaine in pharmaceutical formulations using capillary electrophoresis with spectrophotometric detection (210 nm) is proposed. In this method, the sample preparation was fast and simple, using only aqueous dilution or extraction with sonication aid. A background electrolyte composed by 10 mmol L (-1) of monosodium phosphate with pH 2.1 allowed the separation of the anesthetics with good peak resolution in less than 5 min. The limits of quantification were 0.31 and 0.68 mg L-1 for lidocaine and benzocaine, respectively. The intra- and inter-day precisions (RSD%) ranged from 1.1 to 4.8% and the accuracies (as recovery percentage) varied from 84 to 109.4%. The proposed method was successfully applied for analyses of commercial samples of pharmaceutical formulations, such as otologic solution, ointment, and spray solution and lozenges for sore throat. (AU)

• Processo FAPESP: 14/50867-3 - INCT 2014: Instituto Nacional de Ciência e Tecnologia de Bioanalítica
• Beneficiário: Marco Aurelio Zezzi Arruda
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/06478-3 - Aplicação da Técnica de Impressão Direta 3D na Construção de Dispositivos Microfluídicos
• Beneficiário: José Alberto Fracassi da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/22127-2 - Desenvolvimento de novos materiais estratégicos para dispositivos analíticos integrados
• Beneficiário: Lauro Tatsuo Kubota
• Modalidade de apoio: Auxílio à Pesquisa - Temático