A simple and fast method for determination of benzocaine and lidocaine in pharmaceutical formulations by capillary electrophoresis with spectrophotometric detection

Author(s)

Junger, Ayandra Santos ^[1] ; Souza de Jesus, Francielly Fernanda ^[1] ; Fracassi da Silva, Jose Alberto ^{[1, 2]} ; Daniel, Daniela ^[3] ; de Jesus, Dosil Pereira ^{[1, 2]}

Affiliation

^[1] Univ Estadual Campinas, UNICAMP, Inst Chem, Campinas, SP - Brazil

^[2] Inst Nacl Ciencia & Tecnol Bioanalit, Campinas, SP - Brazil

^[3] Agilent Technol, Barueri, SP - Brazil

• Document type: Journal article
• Source: SEPARATION SCIENCE PLUS; v. 2, n. 11, p. 422-427, NOV 2019.
• Web of Science Citations: 0

Abstract

A novel, simple, and rapid method for the determination of the anesthetics benzocaine and lidocaine in pharmaceutical formulations using capillary electrophoresis with spectrophotometric detection (210 nm) is proposed. In this method, the sample preparation was fast and simple, using only aqueous dilution or extraction with sonication aid. A background electrolyte composed by 10 mmol L (-1) of monosodium phosphate with pH 2.1 allowed the separation of the anesthetics with good peak resolution in less than 5 min. The limits of quantification were 0.31 and 0.68 mg L-1 for lidocaine and benzocaine, respectively. The intra- and inter-day precisions (RSD%) ranged from 1.1 to 4.8% and the accuracies (as recovery percentage) varied from 84 to 109.4%. The proposed method was successfully applied for analyses of commercial samples of pharmaceutical formulations, such as otologic solution, ointment, and spray solution and lozenges for sore throat. (AU)

• FAPESP's process: 14/50867-3 - INCT 2014: National Institute of Science and Technology in Bioanalysis
• Grantee: Marco Aurelio Zezzi Arruda
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/06478-3 - Use of 3D Printing on the Fabrication of Microfluidic Devices
• Grantee: José Alberto Fracassi da Silva
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/22127-2 - Development of novel materials strategic for integrated analytical devices
• Grantee: Lauro Tatsuo Kubota
• Support Opportunities: Research Projects - Thematic Grants