Dendritic Cells Expressing MyD88 Molecule Are Necessary and Sufficient for CpG-Mediated Inhibition of IgE Production In Vivo

Autor(es)

Alberca Custodio, Ricardo W. ^[1] ; Mirotti, Luciana ^[1] ; Gomes, Eliane ^[1] ; Nunes, Fernanda P. B. ^[1] ; Vieira, Raquel S. ^[1] ; Graca, Luis ^[2] ; Almeida, Rafael R. ^[1] ; Camara, Niels O. S. ^[1] ; Russo, Momtchilo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, BR-05508000 Sao Paulo - Brazil

^[2] Univ Lisbon, Inst Mol Med, P-1649028 Lisbon - Portugal

• Tipo de documento: Artigo Científico
• Fonte: CELLS; v. 8, n. 10 OCT 2019.
• Citações Web of Science: 0

Resumo

Elevated levels of immunoglobulin E (IgE) are associated with allergies and other immunological disorders. Sensitization with alum adjuvant favours IgE production while CpG-ODN adjuvant, a synthetic toll-like receptor 9 (TLR9) agonist, inhibits it. The cellular mechanisms underlying in vivo TLR regulation of immunoglobulin production, specially IgE, are still controversial. Specifically, TLR-mediated IgE regulation in vivo is not yet known. In this study we showed that augmented levels of IgE induced by sensitizations to OVA with or without alum adjuvant or with OVA-pulsed dendritic cells (DCs) were inhibited by co-administration of CpG. Notably, CpG-mediated suppression of IgE production required MyD88-expression on DCs but not on B-cells. This finding contrasts with previous in vitro studies reporting regulation of IgE by a direct action of CpG on B cells via MyD88 pathway. In addition, we showed that CpG also inhibited IgE production in a MyD88-dependent manner when sensitization was performed with OVA-pulsed DCs. Finally, CpG signalling through MyD88 pathway was also necessary and sufficient to prevent anaphylactic antibody production involved in active cutaneous anaphylaxis. (AU)

• Processo FAPESP: 15/16728-9 - Imunoterapia na asma experimental por agonistas de receptores toll-like
• Beneficiário: Fernanda Peixoto Barbosa Nunes
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/19906-2 - Perfil de transcrição gênica de células t foliculares auxiliares (Tfh) e mudança isotípica de células b induzida por adjuvantes do tipo 1 ou tipo 2
• Beneficiário: Momtchilo Russo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/24694-1 - Imunoterapia na asma experimental por agonistas de receptores toll-like, infecção ou tolerância
• Beneficiário: Momtchilo Russo
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 16/16602-8 - Imunoterapia na asma experimental por CpG
• Beneficiário: Ricardo Wesley Alberca Custódio
• Modalidade de apoio: Bolsas no Brasil - Doutorado