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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Dendritic Cells Expressing MyD88 Molecule Are Necessary and Sufficient for CpG-Mediated Inhibition of IgE Production In Vivo

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Alberca Custodio, Ricardo W. [1] ; Mirotti, Luciana [1] ; Gomes, Eliane [1] ; Nunes, Fernanda P. B. [1] ; Vieira, Raquel S. [1] ; Graca, Luis [2] ; Almeida, Rafael R. [1] ; Camara, Niels O. S. [1] ; Russo, Momtchilo [1]
Total Authors: 9
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, BR-05508000 Sao Paulo - Brazil
[2] Univ Lisbon, Inst Mol Med, P-1649028 Lisbon - Portugal
Total Affiliations: 2
Document type: Journal article
Source: CELLS; v. 8, n. 10 OCT 2019.
Web of Science Citations: 0

Elevated levels of immunoglobulin E (IgE) are associated with allergies and other immunological disorders. Sensitization with alum adjuvant favours IgE production while CpG-ODN adjuvant, a synthetic toll-like receptor 9 (TLR9) agonist, inhibits it. The cellular mechanisms underlying in vivo TLR regulation of immunoglobulin production, specially IgE, are still controversial. Specifically, TLR-mediated IgE regulation in vivo is not yet known. In this study we showed that augmented levels of IgE induced by sensitizations to OVA with or without alum adjuvant or with OVA-pulsed dendritic cells (DCs) were inhibited by co-administration of CpG. Notably, CpG-mediated suppression of IgE production required MyD88-expression on DCs but not on B-cells. This finding contrasts with previous in vitro studies reporting regulation of IgE by a direct action of CpG on B cells via MyD88 pathway. In addition, we showed that CpG also inhibited IgE production in a MyD88-dependent manner when sensitization was performed with OVA-pulsed DCs. Finally, CpG signalling through MyD88 pathway was also necessary and sufficient to prevent anaphylactic antibody production involved in active cutaneous anaphylaxis. (AU)

FAPESP's process: 15/16728-9 - Immunotherapy of experimental asthma by agonists of toll-like receptors
Grantee:Fernanda Peixoto Barbosa Nunes
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 14/19906-2 - Transcriptional profile of t follicular helper (Tfh) cells and b cell isotype switching induced by type 1 or type 2 adjuvants
Grantee:Momtchilo Russo
Support type: Regular Research Grants
FAPESP's process: 13/24694-1 - Immunotherapy of experimental asthma by agonists of toll-like receptors, infection or tolerance
Grantee:Momtchilo Russo
Support type: Research Projects - Thematic Grants
FAPESP's process: 16/16602-8 - Immunotherapy in experimental asthma by CpG
Grantee:Ricardo Wesley Alberca Custódio
Support type: Scholarships in Brazil - Doctorate