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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Innate Immune Responses Associated with Resistance against Haemonchus contortus in Morada Nova Sheep

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Autor(es):
Barbosa Toscano, Joao Henrique [1] ; Okino, Cintia Hiromi [2] ; dos Santos, Isabella Barbosa [1] ; Giraldelo, Luciana Aparecida [3] ; von Haehling, Marei Borsch [1] ; Esteves, Sergio Novita [2] ; de Souza Chagas, Ana Carolina [2]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista, Fac Ciencias Agr & Vet, Via Acesso Prof Paulo Donato Castellane, BR-14884900 Sao Paulo, SP - Brazil
[2] Embrapa Pecudria Sudeste, Km 234, Fazenda Canchim 339, BR-13560970 Sao Carlos, SP - Brazil
[3] Ctr Univ Cent Paulista, Rua Miguel Petroni 5111, BR-13563470 Sao Carlos, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF IMMUNOLOGY RESEARCH; v. 2019, NOV 11 2019.
Citações Web of Science: 0
Resumo

The immune response against Haemonchus contortus infections is primarily associated with the Th2 profile. However, the exact mechanisms associated with increased sheep resistance against this parasite remains poorly elucidated. The present study is aimed at evaluating mediators from the innate immune response in lambs of the Morada Nova Brazilian breed with contrasting H. contortus resistance phenotypes. Briefly, 287 lambs were characterized through fecal egg counts (FEC) and packed cell volume (PCV) after two independent experimental parasitic challenges with 4,000 H. contortus L-3. 20 extreme resistance phenotypes (10 most resistant and 10 most susceptible) were selected, subjected to a third artificial infection with 4,000 L-3, and euthanized 7 days later. Tissue samples were collected from abomasal fundic and pyloric mucosa and abomasal lymph nodes. Blood samples were collected at days 0 and 7 of the third parasitic challenge. RNA was extracted from tissue and blood samples for relative quantification of innate immune-related genes by RT-qPCR. For the abomasal fundic mucosa, increased TNF alpha and IL1 beta expression levels (P<0.05) were found in the susceptible animals, while resistant animals had IL33 superiorly expressed (P<0.05). Higher levels (P<0.05) of TLR2 and CFI were found in the abomasal pyloric mucosa of resistant animals. TNF alpha was at higher levels (P<0.05) in the blood of susceptible lambs, at day 0 of the third artificial infection. The exacerbated proinflammatory response observed in susceptible animals, at both local and systemic levels, may be a consequence of high H. contortus parasitism. This hypothesis is corroborated by the higher blood levels of TNF alpha before the onset of infection, which probably remained elevated from the previous parasitic challenges. On the other hand, resistant lambs had an enhanced response mediated by TLR recognition and complement activation. Nevertheless, this is the first study to directly associate sheep parasitic resistance with IL33, an innate trigger of the Th2-polarized response. (AU)

Processo FAPESP: 17/24289-0 - Cordeiros Morada Nova: cuidados ao nascimento, avaliação dos níveis de infecção por nematóides gastrintestinais e seu impacto no ganho de peso
Beneficiário:Luciana Aparecida Giraldelo
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 17/01626-1 - Caracterização genética e de respostas imunes associadas ao fenótipo de resistência parasitária em rebanho ovino da raça Morada Nova
Beneficiário:Ana Carolina de Souza Chagas
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 17/00373-2 - Caracterização de respostas imunes locais associadas ao fenótipo de resistência parasitária em cordeiros da raça morada nova
Beneficiário:João Henrique Barbosa Toscano
Linha de fomento: Bolsas no Brasil - Mestrado