Rho-kinase activity is upregulated in the skeletal muscle of aged exercised rats

Autor(es)

Munoz, Vitor Rosetto ^[1] ; Gaspar, Rafael Calais ^[1] ; Minuzzi, Luciele Guerra ^{[1, 2]} ; Canciglieri, Raphael dos Santos ^[1] ; Ramos da Silva, Adelino Sanchez ^{[3, 4]} ; de Moura, Leandro Pereira ^{[1, 5, 6, 7, 8]} ; Cintra, Dennys Esper ^{[5, 9]} ; Ropelle, Eduardo Rochete ^{[1, 5, 7]} ; Pauli, Jose Rodrigo ^{[1, 5, 7]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Lab Mol Biol Exercise, UNICAMP, Limeira, SP - Brazil

^[2] Univ Coimbra, Ctr Neurosci & Cell Biol CNCIBILI, Coimbra - Portugal

^[3] Univ Sao Paulo, Sch Phys Educ & Sport Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Postgrad Program Rehabil & Funct Performance, Ribeirao Preto Med Sch, Ribeirao Preto, SP - Brazil

^[5] Univ Estadual Campinas, OCRC, UNICAMP, Campinas, SP - Brazil

^[6] Sao Paulo State Univ UNESP, Postgrad Program Motor Sci, Sao Paulo, SP - Brazil

^[7] Univ Estadual Campinas, CEPECE Ctr Res Sport Sci, Sch Appl Sci, UNICAMP, Limeira, SP - Brazil

^[8] Univ Estadual Campinas, Exercise Cell Biol Lab, UNICAMP, Limeira, SP - Brazil

^[9] Univ Estadual Campinas, Lab Nutr Genom, UNICAMP, Limeira, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Experimental Gerontology; v. 128, DEC 2019.
• Citações Web of Science: 0

Resumo

There is a gap in the knowledge regarding the regulation of glucose uptake in skeletal muscle during the development of insulin resistance in the elderly. Rho-Kinase (Rock) signaling has been demonstrated as a crucial mechanism related to glucose metabolism and insulin sensitivity in skeletal muscle. This kinase is involved in the insulin receptor substrate 1 (IRS1) phosphorylation, leading to glucose uptake stimulation in the skeletal muscle; however, the mechanisms elucidating the role of Rock regulation in the context of advanced ages are still limited. In this study, we submitted old Fischer 344 rats to short-term treadmill physical exercise protocol (5 days) and evaluated the glucose tolerance and proteins involved with Rock/insulin signaling in the skeletal muscle. Compared to young rats, the old rats showed glucose intolerance, hyperinsulinemia, and decreased phosphorylation in the proteins related to the insulin signaling pathway in the skeletal muscle, without changes in body mass and adiposity. Otherwise, when these rats were submitted to physical exercise, it was found decreased fasting glucose, higher glucose tolerance, decreased insulinemia, and upregulation of Rock2/pIRS1/pAkt/pGSK3 beta/GLUT4 pathway in the skeletal muscle. In summary, the aging process did not change Rock signaling, but the physical exercise was able to increase Rock2 content and insulin signaling pathway in the skeletal muscle. This finding suggests the benefic role of physical exercise to advanced ages, promoting insulin-sensitive effects with Rho-kinase contribution. (AU)

• Processo FAPESP: 18/20872-6 - Efeitos do exercício físico sobre a via de sinalização da proteína Rock e regulação da lipogênese e gliconeogênese no fígado de ratos envelhecidos
• Beneficiário: José Rodrigo Pauli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/26000-2 - O papel da proteína Rock sobre a captação de glicose no músculo esquelético de roedores exercitados durante o processo de envelhecimento
• Beneficiário: Vitor Rosetto Muñoz
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 16/18488-8 - O papel do exercício físico nas vias moleculares de captação de glicose em camundongos nocautes para GLUT-4
• Beneficiário: José Rodrigo Pauli
• Modalidade de apoio: Auxílio à Pesquisa - Regular