| Full text | |
| Author(s): |
Munoz, Vitor Rosetto
[1]
;
Gaspar, Rafael Calais
[1]
;
Minuzzi, Luciele Guerra
[1, 2]
;
Canciglieri, Raphael dos Santos
[1]
;
Ramos da Silva, Adelino Sanchez
[3, 4]
;
de Moura, Leandro Pereira
[1, 5, 6, 7, 8]
;
Cintra, Dennys Esper
[5, 9]
;
Ropelle, Eduardo Rochete
[1, 5, 7]
;
Pauli, Jose Rodrigo
[1, 5, 7]
Total Authors: 9
|
| Affiliation: | [1] Univ Estadual Campinas, Lab Mol Biol Exercise, UNICAMP, Limeira, SP - Brazil
[2] Univ Coimbra, Ctr Neurosci & Cell Biol CNCIBILI, Coimbra - Portugal
[3] Univ Sao Paulo, Sch Phys Educ & Sport Ribeirao Preto, Ribeirao Preto, SP - Brazil
[4] Univ Sao Paulo, Postgrad Program Rehabil & Funct Performance, Ribeirao Preto Med Sch, Ribeirao Preto, SP - Brazil
[5] Univ Estadual Campinas, OCRC, UNICAMP, Campinas, SP - Brazil
[6] Sao Paulo State Univ UNESP, Postgrad Program Motor Sci, Sao Paulo, SP - Brazil
[7] Univ Estadual Campinas, CEPECE Ctr Res Sport Sci, Sch Appl Sci, UNICAMP, Limeira, SP - Brazil
[8] Univ Estadual Campinas, Exercise Cell Biol Lab, UNICAMP, Limeira, SP - Brazil
[9] Univ Estadual Campinas, Lab Nutr Genom, UNICAMP, Limeira, SP - Brazil
Total Affiliations: 9
|
| Document type: | Journal article |
| Source: | Experimental Gerontology; v. 128, DEC 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
There is a gap in the knowledge regarding the regulation of glucose uptake in skeletal muscle during the development of insulin resistance in the elderly. Rho-Kinase (Rock) signaling has been demonstrated as a crucial mechanism related to glucose metabolism and insulin sensitivity in skeletal muscle. This kinase is involved in the insulin receptor substrate 1 (IRS1) phosphorylation, leading to glucose uptake stimulation in the skeletal muscle; however, the mechanisms elucidating the role of Rock regulation in the context of advanced ages are still limited. In this study, we submitted old Fischer 344 rats to short-term treadmill physical exercise protocol (5 days) and evaluated the glucose tolerance and proteins involved with Rock/insulin signaling in the skeletal muscle. Compared to young rats, the old rats showed glucose intolerance, hyperinsulinemia, and decreased phosphorylation in the proteins related to the insulin signaling pathway in the skeletal muscle, without changes in body mass and adiposity. Otherwise, when these rats were submitted to physical exercise, it was found decreased fasting glucose, higher glucose tolerance, decreased insulinemia, and upregulation of Rock2/pIRS1/pAkt/pGSK3 beta/GLUT4 pathway in the skeletal muscle. In summary, the aging process did not change Rock signaling, but the physical exercise was able to increase Rock2 content and insulin signaling pathway in the skeletal muscle. This finding suggests the benefic role of physical exercise to advanced ages, promoting insulin-sensitive effects with Rho-kinase contribution. (AU) | |
| FAPESP's process: | 18/20872-6 - Effects of physical exercise on Rock protein signaling pathway, and lipogenic regulation and gluconeogenesis in the liver of aged rats |
| Grantee: | José Rodrigo Pauli |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 15/26000-2 - The role of Rock protein on glucose uptake in the skeletal muscle of exercised rodents during the aging process |
| Grantee: | Vitor Rosetto Muñoz |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 16/18488-8 - The role of physical exercise on molecular pathways of glucose uptake in GLUT-4 knockout mice |
| Grantee: | José Rodrigo Pauli |
| Support Opportunities: | Regular Research Grants |