Galectin-3 Expression Correlates with Post-surgical Survival in Canine Oral Melanomas

Autor(es)

Vargas, T. H. M. ^[1] ; Pulz, L. H. ^{[2, 1]} ; Ferro, D. G. ^[3] ; Sobral, R. A. ^[4] ; Venturini, M. A. F. A. ^[3] ; Correa, H. L. ^[3] ; Strefezzi, R. F. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Zootecnia & Engn Alimentos, Dept Med Vet, Lab Oncol Comparada & Translac, Pirassununga, SP - Brazil

^[2] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Patol, Sao Paulo, SP - Brazil

^[3] Odontovet Ctr Odontol Vet, Sao Paulo, SP - Brazil

^[4] Onco Cane Vet, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Comparative Pathology; v. 173, p. 49-57, NOV 2019.
• Citações Web of Science: 0

Resumo

Malignant melanomas (MMs) represent 7% of all malignant neoplasms in dogs. Oral melanocytic neoplasms are often malignant and associated with poor prognosis. There are no universally accepted prognostic markers for canine oral melanoma. Galectin (Gal)-3 is a prognostic marker for human neoplasms such as thyroid, gastric, colorectal and prostate cancers. The protein is related to processes that favour cancer progression, such as angiogenesis, proliferation and apoptosis. The aim of the present study was to characterize the immunohistochemical expression of Gal-3 in canine oral melanomas and to compare it with post-surgical survival, the expression of apoptosis-related proteins and other known prognostic tools. Twenty-seven samples of canine oral melanomas were evaluated for Gal-3, B-cell lymphoma (BCL) 2, caspase (CASE) 3 and Ki67 expression, mitotic index and degree of nuclear atypia. Gal-3 cytoplasmic positivity was correlated positively, while nuclear positivity was correlated negatively, with survival. The intensity of BCL2 labelling was also correlated positively with Gal-3 cytoplasmic positivity. Higher nuclear atypia was observed in dogs with melanoma that died due to the tumour, as well as in dogs that survived for <1 year after surgery. We have confirmed the importance of nuclear atypia for MMs and suggest that Gal-3 is a valuable prognostic indicator for this neoplasm. More in-depth studies are needed to unveil Gal-3 functions in canine MMs using larger sample sizes. (C) 2019 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 13/13252-8 - Caracterização de genes relacionados à apoptose em mastocitomas cutâneos caninos e seu valor como indicador prognóstico
• Beneficiário: Ricardo de Francisco Strefezzi
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/11959-2 - Caracterização imuno-histoquímica da galectina-3 como ferramenta prognóstica em melanomas orais caninos
• Beneficiário: Thiago Henrique Moroni Vargas
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 16/03862-1 - Caracterização de fibroblastos associados ao câncer e sua influência sobre neoplasias caninas
• Beneficiário: Ricardo de Francisco Strefezzi
• Modalidade de apoio: Auxílio à Pesquisa - Regular