PpCRN7 and PpCRN20 of Phythophthora parasitica regulate plant cell death leading to enhancement of host susceptibility

Autor(es)

Maximo, Heros J. ^[1] ; Dalio, Ronaldo J. D. ^[1] ; Dias, Renata O. ^[2] ; Litholdo Jr, Celso G. ; Felizatti, Henrique L. ^[3] ; Machado, Marcos A. ^[4]

Afiliação do(s) autor(es)

^[1] Inst Agron IAC, Ctr Citricultura Sylvio Moreira, Biotechnol Lab, Cordeiropolis, SP - Brazil

^[2] Univ Sao Paulo, Inst Quim, Sao Paulo, SP - Brazil

^[3] Univ Estadual Campinas, UNICAMP, Inst Matemat Fis & Comp Cient, Campinas, SP - Brazil

^[4] Litholdo Jr, Jr., Celso G., Inst Agron IAC, Ctr Citricultura Sylvio Moreira, Biotechnol Lab, Cordeiropolis, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BMC PLANT BIOLOGY; v. 19, n. 1 DEC 6 2019.
• Citações Web of Science: 2

Resumo

Background Phytophthora species secrete cytoplasmic effectors from a family named Crinkler (CRN), which are characterised by the presence of conserved specific domains in the N- and C-terminal regions. P. parasitica causes disease in a wide range of host plants, however the role of CRN effectors in these interactions remains unclear. Here, we aimed to: (i) identify candidate CRN encoding genes in P. parasitica genomes; (ii) evaluate the transcriptional expression of PpCRN (Phytophthora parasitica Crinkler candidate) during the P. parasitica interaction with Citrus sunki (high susceptible) and Poncirus trifoliata (resistant); and (iii) functionally characterize two PpCRNs in the model plant Nicotiana benthamiana. Results Our in silico analyses identified 80 putative PpCRN effectors in the genome of P. parasitica isolate `IAC 01/95.1'. Transcriptional analysis revealed differential gene expression of 20 PpCRN candidates during the interaction with the susceptible Citrus sunki and the resistant Poncirus trifoliata. We have also found that P. parasitica is able to recognize different citrus hosts and accordingly modulates PpCRNs expression. Additionally, two PpCRN effectors, namely PpCRN7 and PpCRN20, were further characterized via transient gene expression in N. benthamiana leaves. The elicitin INF-1-induced Hypersensitivity Response (HR) was increased by an additive effect driven by PpCRN7 expression, whereas PpCRN20 expression suppressed HR response in N. benthamiana leaves. Despite contrasting functions related to HR, both effectors increased the susceptibility of plants to P. parasitica. Conclusions PpCRN7 and PpCRN20 have the ability to increase P. parasitica pathogenicity and may play important roles at different stages of infection. These PpCRN-associated mechanisms are now targets of biotechnological studies aiming to break pathogen's virulence and to promote plant resistance. (AU)

• Processo FAPESP: 15/14498-6 - Efetorômica: efetores como novas ferramentas para o controle de Phytophthora parasitica, agente da gomose e podridão de raízes dos citros.
• Beneficiário: Ronaldo José Durigan Dalio
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado