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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Might smoking assuage the pro-inflammatory effect of diabetes in periodontal sites?

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Autor(es):
Miranda, Tamires Szeremeske [1] ; Almeida, Mariana Linhares [1] ; Marins, Leticia Macedo [1] ; Pereira da Silva, Helio Doyle [1] ; Feres, Magda [1] ; Duarte, Poliana Mendes [1, 2]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Guarulhos, Dent Res Div, Dept Periodontol, Sao Paulo - Brazil
[2] Univ Florida, Dept Periodontol, Coll Dent, Gainesville, FL - USA
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: ORAL DISEASES; v. 26, n. 1, p. 200-212, JAN 2020.
Citações Web of Science: 0
Resumo

Objectives This study evaluated the effects of type 2 diabetes mellitus (DM), smoking, and these two factors combined on gingival crevicular fluid levels and ratios of pro-/anti-inflammatory cytokines. Associations between cytokines with each other and with key periodontal pathogens in periodontal sites under the challenge of one or both of these risk factors were also assessed. Methods A total of 102 subjects with periodontitis were included in this cross-sectional study and assigned to one of the following groups: non-diabetic non-smokers (control group, n = 25), non-smokers with DM (DM group, n = 30), non-diabetic smokers (S group, n = 26), and smokers with DM (S + DM group, n = 21). The levels of 13 pro-inflammatory (IFN-gamma, TNF-alpha, MIP-1 alpha, GM-CSF, IL-1 beta, IL-2, IL-6, IL-7, IL-8, IL-12, IL-17, IL-21, and IL-23) and 5 anti-inflammatory (IL-4, IL-5, IL-10, IL-13, and TGF-beta) cytokines were assessed in healthy and diseased sites, using multiplex immunoassay. Ratios of pro-/anti-inflammatory cytokines were obtained in all possible permutations. The levels of 7 key periodontal pathogens were evaluated by qPCR. Results Overall, the ratios of pro-/anti-inflammatory cytokines were higher in healthy and diseased sites of the DM group and in healthy sites of the S + DM group, and lower in diseased sites of the S group, compared with the control (p < .05). The proportion of the pro-inflammatory cytokines in relation to the 18 cytokines studied was higher in the DM group and lower in the S group, whereas the proportion of the anti-inflammatory cytokines was lower in both diabetic groups and higher in the S group, compared to the control (p < .05). A cluster of six common cytokines (IL-4, IL-5, IL-12, IL-13, IL-21, and IL-23) was observed in the diseased sites of all groups studied. Eight common cytokines (IL-4, IL-5, IL-12, IL-13, IL-17, IL-21, IL-23, and IFN-gamma) grouped closely in the healthy sites of both diabetic groups. Significant associations between pathogens and cytokines occurred mainly in the diseased sites of the S + DM group (p < .05). Conclusion Diabetes mellitus induced an overall pro-inflammatory state, while smoking mainly stimulated immunosuppression in periodontal sites. When the two risk factors overlapped, smoking seemed to partially assuage the hyperinflammatory effect of DM. (AU)

Processo FAPESP: 13/23743-9 - Envolvimento dos antagonistas da sinalização Wnt/beta-catenina na periodontite crônica associada a fatores de risco
Beneficiário:Tamires Szeremeske de Miranda
Modalidade de apoio: Bolsas no Brasil - Doutorado
Processo FAPESP: 13/10354-4 - Influência dos fatores de risco para periodontite crônica no perfil imunoinflamatório sistêmico e periodontal
Beneficiário:Poliana Mendes Duarte
Modalidade de apoio: Auxílio à Pesquisa - Regular