Paracoccidioides species present distinct fungal adherence to epithelial lung cells and promote different IL-8 secretion levels

Autor(es)

Almeida, Bruna Rocha ^[1] ; Campitelli Barros, Bianca Carla Silva ^[1] ; Liguori Araujo, Ana Clara ^[1] ; Alcantara, Cristiane ^[1] ; Suzuki, Erika ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, Rua Botucatu, 862 Antonio CM Paiva 6 Andar, BR-04023062 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MEDICAL MICROBIOLOGY AND IMMUNOLOGY; OCT 2019.
• Citações Web of Science: 1

Resumo

Fungi that belong to the genus Paracoccidioides are the etiologic agents of paracoccidioidomycosis, a human systemic mycosis, which occurs in Latin America. Epithelial cell is one of the first cells that interact with these fungi and responds by secreting inflammatory mediators such as cytokines. In the present study, we demonstrate that yeasts of different isolates of Paracoccidioides brasiliensis (Pb18 and Pb03) and Paracoccidioides lutzii (Pb01) distinctly promoted interleukin (IL)-8 secretion by the lung epithelial cell line A549. Depending on the isolate, this cytokine release may rely on the epithelial cell interaction with fungal secreted components or direct contact with the pathogen. In addition, adhesion of yeasts to the pulmonary epithelial cells was also different among Paracoccidioides isolates, and the highest percentage of A549 cells with adhered fungi was observed with P. lutzii. All Paracoccidioides isolates induced an expression increase of alpha 3 and alpha 5 integrins in A549 cells and, using small interfering RNA, we observed that the integrin silencing promoted a reduction of P. lutzii adhesion, which suggests the involvement of integrins in this event. Together, these results indicate that host epithelial cell response may depend on the isolate of Paracoccidioides. (AU)

• Processo FAPESP: 16/06285-5 - Envolvimento de receptores do tipo Toll, integrinas e suas respectivas vias de sinalização celular na secreção de citocinas por células epiteliais durante a infecção com Paracoccidioides Brasiliensis
• Beneficiário: Bianca Carla Silva Campitelli de Barros
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/25652-6 - Estudo da comunicação entre receptores e suas vias de sinalização na secreção de citocinas, induzida por fungos em células epiteliais
• Beneficiário: Erika Suzuki de Toledo
• Linha de fomento: Auxílio à Pesquisa - Regular