Caries removal with Er:YAG laser followed by dentin biomodification with carbodiimide and chitosan: Wettability and surface morphology analysis

Autor(es)

Curylofo-Zotti, Fabiana A. ^[1] ; Fernandes, Maria P. ^[1] ; Martins, Amanda A. ^[1] ; Macedo, Ana Paula ^[2] ; Nogueira, Lucas Fabricio B. ^[3] ; Ramos, Ana Paula ^[3] ; Corona, Silmara A. M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Restorat Dent Dept, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Dept Dent Mat & Prosthodont, Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Chem Dept, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MICROSCOPY RESEARCH AND TECHNIQUE; v. 83, n. 2 OCT 2019.
• Citações Web of Science: 0

Resumo

This study aimed to investigate dentin wettability and surface morphology after selective removal of carious lesion by erbium-doped yttrium aluminum garnet (Er:YAG) laser, followed by dentin biomodification with carbodiimide (EDC) and chitosan (CHI). Seventy-eight bovine dentin specimens were submitted to caries induction. Specimens were distributed according to methods of carious removal (n = 39): bur at low-speed (40,000 rpm) or Er:YAG laser (noncontact mode, 250 mJ/pulse and 4Hz). All specimens were etched with 35% phosphoric acid, and subdivided according to dentin biomodification (n = 13): Control (no biomodification), EDC or CHI. The contact angle (n = 10) between adhesive system (3M ESPE) and dentin surface was measured by a goniometer. Eighteen specimens (n = 3) were analyzed by scanning electron microscopy. Data were analyzed by two-way ANOVA and Tukey's test (alpha = .05). The method used to remove carious lesion did not influence the wettability of dentinal surface (p = .748). The angles produced on the remaining dentin after biomodification were influenced (p = .007). CHI promoted higher contact angles (p = .007) and EDC did not differ from the control group (p = .586). In the bur-treated group, most tubules were open, regardless of which biomodifier was used. Laser modified the organic matrix layer. CHI promoted partially closed tubules in some areas while EDC exposed dentinal tubules. Regardless of which method was used for selective removal of carious lesion, biomodification with EDC did not affect the dentin wettability, whereas CHI changed the wettability of remaining dentin. Both biomodifiers promoted a slight change on dentin morphology. (AU)

• Processo FAPESP: 14/23654-9 - Remoção seletiva de lesão de cárie com Laser de Er:YAG e reumidificação da dentina com solução de quitosana
• Beneficiário: Fabiana Almeida Curylofo Zotti
• Modalidade de apoio: Bolsas no Brasil - Doutorado