Reliable Methods for Analyses of Volatile Compounds of Copaifera Oleoresins Combining Headspace and Gas Chromatography

Autor(es)

Mangabeira da Silva, Jonas Joaquim ^[1] ; Ribeiro, Victor Pena ^[1] ; Lemos, Marivane ^[1] ; Miller Crotti, Antonio Eduardo ^[2] ; Rogez, Herve ^[3] ; Bastos, Jairo Kenupp ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Pharmaceut Sci, Av Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Sch Philosophy Sci & Languages, Chem Dept, Av Bandeirantes 3900, BR-14040903 Ribeirao Preto, SP - Brazil

^[3] Fed Univ Para, Ctr Valorizat Amazonian Bioact Cpds CVACBA, Av Perimetral 01, BR-66075110 Belem, Para - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CHEMISTRY & BIODIVERSITY; v. 17, n. 1 DEC 2019.
• Citações Web of Science: 5

Resumo

Two analytical methods were developed in this study for direct and fast chemical investigation of authentic Copaifera oleoresins (COR) and commercial products. Polydimethylsiloxane microfiber coupled to gas chromatography-mass spectrometry (HS-SPME-GC/MS) showed the best results for oleoresin qualitative analysis, setting the following extraction conditions: equilibrium time of 15 min, extraction time of 30 min, extraction temperature at 60 degrees C and constant stirring of 400 rpm. Sesquiterpenes alpha-copaene, beta-elemene, beta-caryophyllene and trans-alpha-bergamotene were found in all investigated samples. Quantitative analysis by gas chromatography coupled with flame ionization detector (GC-FID) measured the content of the four sesquiterpenes in all samples. Qualitative and quantitative results showed important differences between COR of distinct species and commercial products. Data regarding the volatile composition of C. oblongifolia and C. trapezifolia oleoresins were first presented in this study and two new analytical methods were reported for direct and fast qualitative and quantitative analysis of COR. (AU)

• Processo FAPESP: 11/13630-7 - Validação química e farmacológica de extratos e princípios ativos de espécies de Copaifera
• Beneficiário: Jairo Kenupp Bastos
• Modalidade de apoio: Auxílio à Pesquisa - Temático