Trametes versicolor laccase production using agricultural wastes: a comparative study in Erlenmeyer flasks, bioreactor and tray

Autor(es)

Pinheiro, Vanessa Elisa ^[1] ; Michelin, Michele ^[2] ; Vici, Ana Claudia ^[3] ; de Almeida, Paula Zaghetto ^[1] ; Teixeira de Moraes Polizeli, Maria de Lourdes ^{[3, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Bioquim & Imunol, Fac Med Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[2] Univ Minho, Ctr Biol Engn, Campus Gualtar, Braga - Portugal

^[3] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, Ave Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Bioprocess and Biosystems Engineering; v. 43, n. 3 NOV 2019.
• Citações Web of Science: 2

Resumo

Laccases are very interesting biocatalysts of recognized importance for several industrial applications. Its production by Trametes versicolor, a white-rot fungus, was induced by a combination of cotton gin wastes (1%), a lignocellulosic waste, and vinasse (15%), an industrial by-product from sugarcane industry. The use of these agro-industrial wastes are interesting, since it helps in reducing the enzyme production costs, due to their low cost and wide availability, as well as the environmental contamination issues, due to their improper disposal. Thus, laccase production was studied in submerged fermentation of T. versicolor using these agro-industrial wastes (cotton gin waste and vinasse) as carbon source and an additional nitrogen source (0.1% peptone). Three different bioreactors were evaluated for laccase production, such as BioFlo 310 bioreactor, aluminium tray and Erlenmeyer flasks to achieve high levels of laccase production. The highest specific production of laccase was found in BioFlo 310 bioreactor with 12 days of fermentation (55.24 U/mg prot.), which has been shown to be closely related to the oxygen supply to the microorganism through aeration of the fermentation medium. This study brings new insights into green biotechnology regarding vinasse utilization, which is frequently discharged in soils, rivers, and lakes causing adverse effects on agricultural soils and biota, as well as the cotton gin waste recovery. (AU)

• Processo FAPESP: 14/50884-5 - INCT 2014: Instituto Nacional de Ciência e Tecnologia do Bioetanol
• Beneficiário: Marcos Silveira Buckeridge
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/23200-0 - Produção de coquetéis enzimáticos com potencial no biobranqueamento da polpa de celulose para a fabricação de papel a partir de resíduos lignocelulósicos e fibras secundárias
• Beneficiário: Vanessa Elisa Pinheiro
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 10/52322-3 - Bioprospecção de fungos filamentosos produtores de holoenzimas com aplicação em biorefinaria
• Beneficiário: Maria de Lourdes Teixeira de Moraes Polizeli
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular