Resistance to triazole fungicides in Pyricularia species is associated with invasive plants from wheat fields in Brazil

Autor(es)

Dorigan, Adriano Francis ^[1] ; de Carvalho, Giselle ^[2] ; Poloni, Nadia Maria ^[3] ; Negrisoli, Matheus Mereb ^[4] ; Nunes Maciel, Joao Leodato ^[5] ; Ceresini, Paulo Cezar ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Lavras, Campus Univ, Lavras, MG - Brazil

^[2] Univ Estadual Paulista, Campus Ilha Solteira, Ilha Solteira, SP - Brazil

^[3] Univ Estadual Paulista, Campus Jaboticabal, Jaboticabal, SP - Brazil

^[4] Univ Estadual Paulista, Campus Botucatu, Botucatu, SP - Brazil

^[5] Empresa Brasileira Pesquisa Agr, Ctr Nacl Pesquisa Trigo, Passo Fundo, RS - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Acta Scientiarum. Agronomy; v. 41, 2019.
• Citações Web of Science: 0

Resumo

Triazole fungicides have not been effective for managing the wheat blast disease in Brazil. A broad analysis across six geographical populations of Pyricularia graminis-tritici in central-southern Brazil indicated a high level of resistance to triazole fungicides. Since P. graminis-tritici is also associated with others poaceous species, here, we analyzed whether triazole-resistant isolates of the blast pathogen could be recovered from other poaceous hosts that are invasive of sprayed wheat fields. In addition to P. graminis-tritici (Pygt), we also evaluated the levels of sensitivity of three other grass-associated blast pathogens, which included P. grisea (Pg), P. pennisetigena (Pp), and P. urashimae (Pu). Resistance to the triazole fungicides tebuconazole and epoxiconazole was assessed phenotypically based on EC50 values and molecularly by analysis of the presence of mutations in the CYP51A gene, which encodes for the target enzyme 14-alpha-demethylase. We detected triazole-resistant Pyricularia spp. (Pg, Pp, Pu and Pygt) that is associated with Avena sativa, Cenchrus echinatus, Chloris distichophylla, Cynodon sp., Digitaria horizontalis, D. sanguinalis, Panicum maximum or Urochloa spp. The major outcome from our study was the evidence that invasive poaceous species from wheat fields could be an important source of triazole resistant fungal inoculum for the initial phases of the wheat blast epidemics. (AU)

• Processo FAPESP: 17/50456-1 - Compreendendo a evolução para resistência a fungicidas em populações de campo do patógeno da brusone do trigo no Brasil: é possível aprender lições para o futuro manejo da doença?
• Beneficiário: Paulo Cezar Ceresini
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/10453-8 - A emergência de Pyricularia tritici sp. Nov. como patógeno da brusone do trigo no Brasil: especiação simpátrica inferida por filogenia multiloci, espectro de patogenicidade e evolução de genes de virulência
• Beneficiário: Paulo Cezar Ceresini
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/10655-4 - Desvendando a origem local da brusone do trigo (Magnaporthe oryzae) no Centro Sul do Brasil e evidências da emergência de resistência a fungicidas estrobilurinas em populações do patógeno
• Beneficiário: Paulo Cezar Ceresini
• Modalidade de apoio: Auxílio à Pesquisa - Regular