Triiodothyronine activated extranuclear pathways upregulate adiponectin and leptin in murine adipocytes

Autor(es)

Mathias, Lucas Solla ^[1] ; Rodrigues, Bruna Moretto ^[1] ; Goncalves, Bianca Mariani ^[1] ; Fontes Moretto, Fernanda Cristina ^[1] ; Castro Olimpio, Regiane Marques ^[1] ; Depra, Igor ^[1] ; De Sibio, Maria Teresa ^[1] ; Tilli, Helena Paim ^[1] ; Nogueira, Celia Regina ^[1] ; de Oliveira, Miriane ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, UNESP, Botucatu Med Sch, Dist Rubido Jr S-N, BR-18618000 Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Molecular and Cellular Endocrinology; v. 503, MAR 1 2020.
• Citações Web of Science: 0

Resumo

Adiponectin and leptin, important for metabolic regulation, are synthesized and secreted by adipose tissue and are influenced by triiodothyronine (T3) that activates the MAPK/ERK and integrin alpha V beta 3 pathways, modulating gene expression. Adipocytes were treated with T3 (10 nM), for 1 h, in the absence or presence of PD98059 (PD) and tetraiodothyroacetic acid (Tetrac), which are pathways inhibitors. The cells were incubated with Adipo Red/Oil Red O reagents, and intracellular lipid accumulation {[}glycerol and triacylglycerol (TAG)], MTT, 8-hydroxideoxyguanosine (8-OH-dG), and mRNA and protein expression were assessed. T3 increased leptin mRNA and protein expression, and, in contrast, there was a decrease in the Tetrac + T3 group. Adiponectin mRNA expression was not altered by T3, though it had increased its protein expression, which was terminated by inhibitors PD + T3 and Tetrac + T3. However, T3 did not alter PPAR gamma protein expression, lipid accumulation, TAG, glycerol, and DNA damage, but PD + T3 and Tetrac + T3 reduced these parameters. T3 activated the MAPK/ERK pathway on adipocytes to modulate the adiponectin protein expression and integrin alpha V beta 3 to alter the leptin gene expression. (AU)

• Processo FAPESP: 10/16911-4 - Ação genômica e não genômica da triiodotironina (t3) na expressão gênica e protéica da leptina e adiponectina em células 3t3-l1
• Beneficiário: Celia Regina Nogueira
• Modalidade de apoio: Auxílio à Pesquisa - Regular