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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Triiodothyronine activated extranuclear pathways upregulate adiponectin and leptin in murine adipocytes

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Author(s):
Mathias, Lucas Solla [1] ; Rodrigues, Bruna Moretto [1] ; Goncalves, Bianca Mariani [1] ; Fontes Moretto, Fernanda Cristina [1] ; Castro Olimpio, Regiane Marques [1] ; Depra, Igor [1] ; De Sibio, Maria Teresa [1] ; Tilli, Helena Paim [1] ; Nogueira, Celia Regina [1] ; de Oliveira, Miriane [1]
Total Authors: 10
Affiliation:
[1] Sao Paulo State Univ, UNESP, Botucatu Med Sch, Dist Rubido Jr S-N, BR-18618000 Botucatu, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Molecular and Cellular Endocrinology; v. 503, MAR 1 2020.
Web of Science Citations: 0
Abstract

Adiponectin and leptin, important for metabolic regulation, are synthesized and secreted by adipose tissue and are influenced by triiodothyronine (T3) that activates the MAPK/ERK and integrin alpha V beta 3 pathways, modulating gene expression. Adipocytes were treated with T3 (10 nM), for 1 h, in the absence or presence of PD98059 (PD) and tetraiodothyroacetic acid (Tetrac), which are pathways inhibitors. The cells were incubated with Adipo Red/Oil Red O reagents, and intracellular lipid accumulation {[}glycerol and triacylglycerol (TAG)], MTT, 8-hydroxideoxyguanosine (8-OH-dG), and mRNA and protein expression were assessed. T3 increased leptin mRNA and protein expression, and, in contrast, there was a decrease in the Tetrac + T3 group. Adiponectin mRNA expression was not altered by T3, though it had increased its protein expression, which was terminated by inhibitors PD + T3 and Tetrac + T3. However, T3 did not alter PPAR gamma protein expression, lipid accumulation, TAG, glycerol, and DNA damage, but PD + T3 and Tetrac + T3 reduced these parameters. T3 activated the MAPK/ERK pathway on adipocytes to modulate the adiponectin protein expression and integrin alpha V beta 3 to alter the leptin gene expression. (AU)

FAPESP's process: 10/16911-4 - GENOMIC AND NON GENOMIC ACTION OF TRIIODOTIRONINE (T3) IN THE GENE EXPRESSION AND LEPTIN AND ADIPONECTIN PROTEIN IN CELLS 3T3-L1
Grantee:Celia Regina Nogueira
Support Opportunities: Regular Research Grants