Simple and Rapid Method by Ultra High-Performance Liquid Chromatography (UHPLC) with Ultraviolet Detection for Determination of Efavirenz in Plasma: Application in a Preclinical Pharmacokinetic Study

Autor(es)

Martins, Evelin dos Santos ^[1] ; Oliveira, Jonata Augusto ^[1] ; Franchin, Taisa Busaranho ^[1] ; Ulian Silva, Bruna Cristina ^[1] ; Candido, Caroline Damico ^[1] ; Peccinini, Rosangela Goncalves ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista Julio de Mesquita Filho UN, Dept Nat Act Principles & Toxicol, Sch Pharmaceut Sci, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Chromatographic Science; v. 57, n. 10, p. 874-880, NOV 2019.
• Citações Web of Science: 0

Resumo

A simple and rapid ultra-high-performance liquid chromatography (UHPLC) method for determination of efavirenz (EFV) in plasma was developed and applied in a preclinical pharmacokinetic study. The method involves only addition of acetonitrile to precipitation of plasma proteins followed by solvent evaporation. The mobile phase consisted of methanol, acetonitrile and 0.1 M formic acid (20:50:30) at a flow rate of 0.3 mL/min with run time of 5 min. A CSH C18 column and a UHPLC-UV system operating at 245 nm were used. There was a linear response in the range of 0.078 to 10 mu g/mL, and the equation was obtained by weighting (1/x(2)) with r(2) = 0.9965. The pharmacokinetic disposition of EFV was investigated in rabbits (two groups, n = 7) following a single intravenous administration (IV group) at a dose of 2.7 mg/kg and a single oral administration (oral group) of EFV co-administered with lamivudine (3TC) and tenofovir (TNF) at a dose of 50, 25 and 25 mg, respectively. The study demonstrated the applicability of the method for determination of EFV in plasma without interference from other co-administered drugs, and the pharmacokinetic parameters were calculated. The method showed advantages over other methods in the literature, such as simplicity of sample processing and fast results. (AU)

• Processo FAPESP: 11/11239-9 - Farmacocinética pré-clínica de novos fármacos e medicamentos
• Beneficiário: Rosangela Gonçalves Peccinini
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/23843-9 - Farmacocinética do efavirenz administrado na forma de nanoparticulas inorganicas em coelhos
• Beneficiário: Evelin dos Santos Martins
• Modalidade de apoio: Bolsas no Brasil - Mestrado