| Full text | |
| Author(s): |
Martins, Evelin dos Santos
[1]
;
Oliveira, Jonata Augusto
[1]
;
Franchin, Taisa Busaranho
[1]
;
Ulian Silva, Bruna Cristina
[1]
;
Candido, Caroline Damico
[1]
;
Peccinini, Rosangela Goncalves
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Estadual Paulista Julio de Mesquita Filho UN, Dept Nat Act Principles & Toxicol, Sch Pharmaceut Sci, Sao Paulo - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | Journal of Chromatographic Science; v. 57, n. 10, p. 874-880, NOV 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
A simple and rapid ultra-high-performance liquid chromatography (UHPLC) method for determination of efavirenz (EFV) in plasma was developed and applied in a preclinical pharmacokinetic study. The method involves only addition of acetonitrile to precipitation of plasma proteins followed by solvent evaporation. The mobile phase consisted of methanol, acetonitrile and 0.1 M formic acid (20:50:30) at a flow rate of 0.3 mL/min with run time of 5 min. A CSH C18 column and a UHPLC-UV system operating at 245 nm were used. There was a linear response in the range of 0.078 to 10 mu g/mL, and the equation was obtained by weighting (1/x(2)) with r(2) = 0.9965. The pharmacokinetic disposition of EFV was investigated in rabbits (two groups, n = 7) following a single intravenous administration (IV group) at a dose of 2.7 mg/kg and a single oral administration (oral group) of EFV co-administered with lamivudine (3TC) and tenofovir (TNF) at a dose of 50, 25 and 25 mg, respectively. The study demonstrated the applicability of the method for determination of EFV in plasma without interference from other co-administered drugs, and the pharmacokinetic parameters were calculated. The method showed advantages over other methods in the literature, such as simplicity of sample processing and fast results. (AU) | |
| FAPESP's process: | 11/11239-9 - Preclinical pharmacokinetics of new drugs and pharmaceuticals forms |
| Grantee: | Rosangela Gonçalves Peccinini |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 15/23843-9 - Pharmacokinetics of efavirenz administered in the form of inorganic nanoparticles in rabbits |
| Grantee: | Evelin dos Santos Martins |
| Support Opportunities: | Scholarships in Brazil - Master |